4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
23 Jul 2024

Historique:

received: 13 09 2023

accepted: 14 07 2024

medline: 24 7 2024

pubmed: 24 7 2024

entrez: 23 7 2024

Statut: epublish

Résumé

Single-particle tracking techniques enable investigation of the complex functions and interactions of individual particles in biological environments. Many such techniques exist, each demonstrating trade-offs between spatiotemporal resolution, spatial and temporal range, technical complexity, and information content. To mitigate these trade-offs, we enhanced a confocal laser scanning microscope with an asynchronous read-out single-photon avalanche diode array detector. This detector provides an image of the particle's emission, precisely reflecting its position within the excitation volume. This localization is utilized in a real-time feedback system to drive the microscope scanning mechanism and ensure the particle remains centered inside the excitation volume. As each pixel is an independent single-photon detector, single-particle tracking is combined with fluorescence lifetime measurement. Our system achieves 40 nm lateral and 60 nm axial localization precision with 100 photons and sub-millisecond temporal sampling for real-time tracking. Offline tracking can refine this precision to the microsecond scale. We validated the system's spatiotemporal resolution by tracking fluorescent beads with diffusion coefficients up to 10 μm

Identifiants

DOI: 10.1038/s41467-024-50512-9 PMID: 39043637

pubmed: 39043637

doi: 10.1038/s41467-024-50512-9

pii: 10.1038/s41467-024-50512-9

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

6188

Subventions

Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)

ID : 818699

ID : 855923

Organisme : Associazione Italiana per la Ricerca sul Cancro (Italian Association for Cancer Research)

ID : 23053

Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca (Ministry of Education, University and Research)

ID : 2017P352Z4

ID : 818699

Informations de copyright

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4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.

Journal

Informations de publication

Résumé

Identifiants

Types de publication

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Pagination

Subventions

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Références

Auteurs

Andrea Bucci (A)

Giorgio Tortarolo (G)

Marcus Oliver Held (MO)

Luca Bega (L)

Eleonora Perego (E)

Francesco Castagnetti (F)

Irene Bozzoni (I)

Eli Slenders (E)

Giuseppe Vicidomini (G)

Classifications MeSH