B cells secrete functional antigen-specific IgG antibodies on extracellular vesicles.
Animals
Extracellular Vesicles
/ immunology
Immunoglobulin G
/ immunology
Mice
Mice, Transgenic
B-Lymphocytes
/ immunology
Orthomyxoviridae Infections
/ immunology
Green Fluorescent Proteins
/ metabolism
Antigens
/ immunology
Immunoglobulin Class Switching
Mice, Inbred C57BL
Germinal Center
/ immunology
Adaptive immunity
Antibody production
B cells
Extracellular vesicles
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 Jul 2024
23 Jul 2024
Historique:
received:
24
01
2024
accepted:
17
07
2024
medline:
24
7
2024
pubmed:
24
7
2024
entrez:
23
7
2024
Statut:
epublish
Résumé
B cells and the antibodies they produce are critical in host defense against pathogens and contribute to various immune-mediated diseases. B cells responding to activating signals in vitro release extracellular vesicles (EV) that carry surface antibodies, yet B cell production of EVs that express antibodies and their function in vivo is incompletely understood. Using transgenic mice expressing the Cre recombinase in B cells switching to IgG1 to induce expression of fusion proteins between emerald green fluorescent protein (emGFP) and the EV tetraspanin CD63 as a model, we identify emGFP expression in B cells responding to foreign antigen in vivo and characterize the emGFP
Identifiants
pubmed: 39043800
doi: 10.1038/s41598-024-67912-y
pii: 10.1038/s41598-024-67912-y
doi:
Substances chimiques
Immunoglobulin G
0
Green Fluorescent Proteins
147336-22-9
Antigens
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
16970Subventions
Organisme : NIH HHS
ID : AI147394, AG069264, AI112844, HL170961, AI176171, and AI154598
Pays : United States
Organisme : NIH HHS
ID : AI153990
Pays : United States
Informations de copyright
© 2024. The Author(s).
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