Effects of proanthocyanidin-functionalized hydroxyapatite nanoparticles on dentin bonding.
Proanthocyanidins
/ chemistry
Durapatite
/ chemistry
Nanoparticles
/ chemistry
Tensile Strength
Humans
Dental Bonding
/ methods
Materials Testing
Dentin-Bonding Agents
/ chemistry
Dentin
Surface Properties
In Vitro Techniques
Dental Stress Analysis
Hydrogen-Ion Concentration
Composite Resins
/ chemistry
Random Allocation
Collagen cross-linkers
Dentin biomodification
MMPs
Microtensile bond strength
Nanomaterials
Zymography
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
24 Jul 2024
24 Jul 2024
Historique:
received:
07
05
2024
accepted:
17
07
2024
medline:
24
7
2024
pubmed:
24
7
2024
entrez:
24
7
2024
Statut:
epublish
Résumé
To evaluate the effect of proanthocyanidin-functionalized hydroxyapatite nanoparticles (nHAp_PA) used as pretreatment at different concentrations on the microtensile bond strength (µTBS) and endogenous enzymatic activity (MMPs) on pH-cycled dentin after 24 h and 6 months of artificial aging. Fifty human sound dentin blocks were randomly assigned to 5 groups (n = 10): (i) negative control (no treatment); (ii) positive control (pH-cycling); (iii) pH-cycling + 2% nHAp_PA for 60s; (iv) pH-cycling + 6.5% nHAp_PA for 60s; (v) pH-cycling + 15% nHAp_PA for 60s. A self-etch adhesive was used for bonding procedures before resin composite build-ups. Specimens were tested with the µTBS test after 24 h and 6 months of laboratory storage. The proteolytic activity in each group was evaluated with gelatin zymography and in situ zymography. Data were statistically analyzed (p < 0.05). At 24 h, the µTBS of the experimental groups were significantly higher than the controls (p ≤ 0.001), and no differences were observed between different concentrations (p > 0.05). Artificial aging significantly decreased bond strength in all groups (p ≤ 0.008); however, nHAp_PA 2% still yielded higher bonding values than controls (p ≤ 0.007). The groups pretreated with nHAp_PA exhibited lower MMP-9 and MMP-2 activities compared to the positive control group and almost the same enzymatic activity as the negative control group. In situ zymography showed that after 6 months of aging, nHAp_PA 2% and nHAp_PA 6,5% decreased enzymatic activity as well as the negative control. Dentin pretreatment with nHAp_PA increased the bonding performance of a self-etch adhesive and decreased MMP-2 and MMP-9 activities after 6 months.
Identifiants
pubmed: 39046575
doi: 10.1007/s00784-024-05836-7
pii: 10.1007/s00784-024-05836-7
doi:
Substances chimiques
Proanthocyanidins
0
Durapatite
91D9GV0Z28
proanthocyanidin
18206-61-6
Dentin-Bonding Agents
0
Composite Resins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
444Informations de copyright
© 2024. The Author(s).
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