Regional invasion history and land use shape the prevalence of non-native species in local assemblages.
biological invasion
invasion debt
land use
local assemblages
residence time
Journal
Global change biology
ISSN: 1365-2486
Titre abrégé: Glob Chang Biol
Pays: England
ID NLM: 9888746
Informations de publication
Date de publication:
Jul 2024
Jul 2024
Historique:
revised:
26
06
2024
received:
14
03
2024
accepted:
03
07
2024
medline:
26
7
2024
pubmed:
26
7
2024
entrez:
25
7
2024
Statut:
ppublish
Résumé
The ecological impact of non-native species arises from their establishment in local assemblages. However, the rates of non-native spread in new regions and their determinants have not been comprehensively studied. Here, we combined global databases documenting the occurrence of non-native species and residence of non-native birds, mammals, and vascular plants at regional and local scales to describe how the likelihood of non-native occurrence and their proportion in local assemblages relate with their residence time and levels of human usage in different ecosystems. Our findings reveal that local non-native occurrence generally increases with residence time. Colonization is most rapid in croplands and urban areas, while it is slower and variable in natural or semi-natural ecosystems. Notably, non-native occurrence continues to rise even 200 years after introduction, especially for birds and vascular plants, and in other land-use types rather than croplands and urban areas. The impact of residence time on non-native proportions is significant only for mammals. We conclude that the continental exchange of biotas requires considerable time for effects to manifest at the local scale across taxa and land-use types. The unpredictability of future impacts, implied by the slow spread of non-native species, strengthens the call for stronger regulations on the exchange of non-native species to reduce the long-lasting invasion debt looming on ecosystems' future.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e17426Subventions
Organisme : Austrian Science Found (FWF)
ID : M2714-B29
Organisme : Akademie Věd České Republiky
ID : 19-28807X
Organisme : Austrian Science Foundation FWF
ID : I2086-B29
Organisme : Foundation for Science and Technology (FCT)
ID : PTDC/BIA-ECO/0207/2020
Organisme : Foundation for Science and Technology (FCT)
ID : UIDP/00295/2020
Organisme : Foundation for Science and Technology (FCT)
ID : UIDB/00295/2020
Organisme : German Research Foundation DFG
ID : 264740629
Organisme : German Research Foundation DFG
ID : FZT 118 202548816
Organisme : Czech Academy of Sciences
ID : RVO 67985939
Organisme : Grantová Agentura České Republiky
ID : 19-28807X
Informations de copyright
© 2024 The Author(s). Global Change Biology published by John Wiley & Sons Ltd.
Références
Alexander, J. M., Kueffer, C., Daehler, C. C., Edwards, P. J., Pauchard, A., Seipel, T., Arévalo, J., Cavieres, L., Dietz, H., Jakobs, G., McDougall, K., Naylor, B., Otto, R., Parks, C. G., Rew, L., & Walsh, N. (2011). Assembly of nonnative floras along elevational gradients explained by directional ecological filtering. Proceedings of the National Academy of Sciences of the United States of America, 108(2), 656–661. https://doi.org/10.1073/pnas.1013136108
Alexander, J. M., & Levine, J. M. (2019). Earlier phenology of a nonnative plant increases impacts on native competitors. Proceedings of the National Academy of Sciences of the United States of America, 116(13), 6199–6204. https://doi.org/10.1073/pnas.1820569116
Aronson, M. F. J., La Sorte, F. A., Nilon, C. H., Katti, M., Goddard, M. A., Lepczyk, C. A., Warren, P. S., Williams, N. S. G., Cilliers, S., Clarkson, B., Dobbs, C., Dolan, R., Hedblom, M., Klotz, S., Kooijmans, J. L., Kühn, I., Macgregor‐Fors, I., Mcdonnell, M., Mörtberg, U., … Winter, M. (2014). A global analysis of the impacts of urbanization on bird and plant diversity reveals key anthropogenic drivers. Proceedings of the Royal Society B: Biological Sciences, 281(1780), 20133330. https://doi.org/10.1098/rspb.2013.3330
Banki, O., Hobern, D., Döring, M., & Remsen, D. (2019). Catalogue of life plus: A collaborative project to complete the checklist of the world's species. Biodiversity Information Science and Standards, 3, e37652. https://doi.org/10.3897/biss.3.37652
Bates, D., Maechler, M., Bolker, B., Walker, S., Christensen, R. H. B., Singmann, H., & Bolker, M. B. (2015). Lme4. In Convergence.
Biancolini, D., Vascellari, V., Melone, B., Blackburn, T. M., Cassey, P., Scrivens, S. L., & Rondinini, C. (2021). DAMA: The global distribution of alien mammals database. Ecology, 102(11), e03474. https://doi.org/10.1002/ecy.3474
Brooks, M. E., Bolker, B., Kristensen, K., Maechler, M., Magnusson, A., McGillycuddy, M., Skaug, H., Nielsen, A., Berg, C., van Bentham, K., Sadat, N., Lüdecke, D., Lenth, R., O'Brien, J., Geyer, C. J., & Jagan, M. (2022). Package “glmmTMB.”. https://orcid.org/0000‐0001‐9683‐9262
Brummitt, R. K. (2001). World geographical scheme for recording plant distributions edition 2. Group (Issue August) https://doi.org/10.1043/0363‐6445‐28.4.807
Capinha, C., Essl, F., Porto, M., & Seebens, H. (2023). The worldwide networks of spread of recorded alien species. Proceedings of the National Academy of Sciences of the United States of America, 120(1), e2201911120. https://doi.org/10.1073/pnas.2201911120
Chytrý, M., Jarošík, V., Pyšek, P., Hájek, O., Knollová, I., Tichý, L., & Danihelka, J. (2008). Separating habitat invasibility by alien plants from the actual level of invasion. Ecology, 89(6), 1541–1553. https://doi.org/10.1890/07‐0682.1
Chytrý, M., Maskell, L. C., Pino, J., Pyšek, P., Vilà, M., Font, X., & Smart, S. M. (2008). Habitat invasions by alien plants: A quantitative comparison among Mediterranean, subcontinental and oceanic regions of Europe. Journal of Applied Ecology, 45(2), 448–458. https://doi.org/10.1111/j.1365‐2664.2007.01398.x
Chytrý, M., Pyšek, P., Wild, J., Pino, J., Maskell, L. C., & Vila, M. (2009). Europea map of alien plant invasions based on the quantitative assessment across habitats. Diversity and Distributions, 21(1), 1215–1221.
Clout, M. N., & Russell, J. C. (2008). The invasion ecology of mammals: A global perspective. Wildlife Research, 35(3), 180–184. https://doi.org/10.1071/WR07091
Cox, D. R. (1972). Regression models and life‐tables. Journal of the Royal Statistical Society: Series B: Methodological, 34(2), 187–202. https://about.jstor.org/terms
Daly, E. Z., Chabrerie, O., Massol, F., Facon, B., Hess, M. C. M., Tasiemski, A., Grandjean, F., Chauvat, M., Viard, F., Forey, E., Folcher, L., Buisson, E., Boivin, T., Baltora‐Rosset, S., Ulmer, R., Gibert, P., Thiébaut, G., Pantel, J. H., Heger, T., … Renault, D. (2023). A synthesis of biological invasion hypotheses associated with the introduction–naturalisation–invasion continuum. Oikos, 2023(5), e09645. https://doi.org/10.1111/oik.09645
Daru, B. H., Davies, T. J., Willis, C. G., Meineke, E. K., Ronk, A., Zobel, M., Pärtel, M., Antonelli, A., & Davis, C. C. (2021). Widespread homogenization of plant communities in the Anthropocene. Nature Communications, 12(1), 6983. https://doi.org/10.1038/s41467‐021‐27186‐8
Davis, M. A., & Thompson, K. (2000). Eight ways to be a colonizer; two ways to Be an invader: A proposed nomenclature scheme for invasion ecology. Bulletin of the Ecological Society of America, 81(3), 226‐230.
Dawson, W., Moser, D., Van Kleunen, M., Kreft, H., Pergl, J., Pyšek, P., Weigelt, P., Winter, M., Lenzner, B., Blackburn, T. M., Dyer, E. E., Cassey, P., Scrivens, S. L., Economo, E. P., Guénard, B., Capinha, C., Seebens, H., García‐Díaz, P., Nentwig, W., … Essl, F. (2017). Global hotspots and correlates of alien species richness across taxonomic groups. Nature Ecology & Evolution, 1(JUNE), 1–7. https://doi.org/10.1038/s41559‐017‐0186
Diagne, C., Leroy, B., Vaissière, A. C., Gozlan, R. E., Roiz, D., Jarić, I., Salles, J. M., Bradshaw, C. J. A., & Courchamp, F. (2021). High and rising economic costs of biological invasions worldwide. Nature, 592(7855), 571–576. https://doi.org/10.1038/s41586‐021‐03405‐6
Didham, R. K., Tylianakis, J. M., Gemmell, N. J., Rand, T. A., & Ewers, R. M. (2007). Interactive effects of habitat modification and species invasion on native species decline. Trends in Ecology & Evolution, 22(9), 489–496. https://doi.org/10.1016/j.tree.2007.07.001
Duncan, R. P. (2021). Time lags and the invasion debt in plant naturalisations. Ecology Letters, 24(7), 1363–1374. https://doi.org/10.1111/ele.13751
Dyer, E. E., Cassey, P., Redding, D. W., Collen, B., Franks, V., Gaston, K. J., Jones, K. E., Kark, S., Orme, C. D. L., & Blackburn, T. M. (2017). The global distribution and drivers of alien bird species richness. PLoS Biology, 15(1), e2000942. https://doi.org/10.1371/journal.pbio.2000942
Dyer, E. E., Franks, V., Cassey, P., Collen, B., Cope, R. C., Jones, K. E., Şekercioğlu, Ç. H., & Blackburn, T. M. (2016). A global analysis of the determinants of alien geographical range size in birds. Global Ecology and Biogeography, 25(11), 1346–1355. https://doi.org/10.1111/geb.12496
Essl, F., Dullinger, S., Rabitsch, W., Hulme, P. E., Hulber, K., Jarosik, V., Kleinbauer, I., Krausmann, F., Kuhn, I., Nentwig, W., Vila, M., Genovesi, P., Gherardi, F., Desprez‐Loustau, M.‐L., Roques, A., & Pysek, P. (2011). Socioeconomic legacy yields++ an invasion debt. Proceedings of the National Academy of Sciences of the United States of America, 108(1), 203–207. https://doi.org/10.1073/pnas.1011728108
Essl, F., Latombe, G., Lenzner, B., Wilson, J. R. U., Genovesi, P., Pagad, S., Seebens, H., & Smith, K. (2020). The convention on biological diversity (CBD)'s Post‐2020 target on invasive alien species—What should it include and how should it be monitored? NeoBiota, 62, 99–121. https://doi.org/10.3897/neobiota.62.53972
Flores‐Moreno, H., Reich, P. B., Lind, E. M., Sullivan, L. L., Seabloom, E. W., Yahdjian, L., MacDougall, A. S., Reichmann, L. G., Alberti, J., Báez, S., Bakker, J. D., Cadotte, M. W., Caldeira, M. C., Chaneton, E. J., D'Antonio, C. M., Fay, P. A., Firn, J., Hagenah, N., Harpole, W. S., … Borer, E. T. (2016). Climate modifies response of non‐native and native species richness to nutrient enrichment. Philosophical Transactions of the Royal Society, B: Biological Sciences, 371(1694), 20150273. https://doi.org/10.1098/rstb.2015.0273
Gioria, M., Hulme, P. E., Richardson, D. M., & Pyšek, P. (2023). Why are invasive plants successful? Annual Review of Plant Biology, 2023(74), 635–670. https://doi.org/10.1146/annurev‐arplant‐070522
Goel, M. K., Khanna, P., & Kishore, J. (2010). Understanding survival analysis: Kaplan‐Meier estimate. International Journal of Ayurveda Research, 1(4), 274. https://doi.org/10.4103/0974‐7788.76794
Hejda, M., Chytrý, M., Pergl, J., & Pyšek, P. (2015). Native‐range habitats of invasive plants: Are they similar to invaded‐range habitats and do they differ according to the geographical direction of invasion? Diversity and Distributions, 21(3), 312–321. https://doi.org/10.1111/ddi.12269
Hudson, L. N., Newbold, T., Contu, S., Hill, S. L. L., Lysenko, I., De Palma, A., Phillips, H. R. P., Alhusseini, T. I., Bedford, F. E., Bennett, D. J., Booth, H., Burton, V. J., Chng, C. W. T., Choimes, A., Correia, D. L. P., Day, J., Echeverría‐Londoño, S., Emerson, S. R., Gao, D., … Purvis, A. (2017). The database of the PREDICTS (projecting responses of ecological diversity in changing terrestrial systems) project. Ecology and Evolution, 7(1), 145–188. https://doi.org/10.1002/ece3.2579
Hudson, L. N., Newbold, T., Contu, S., Hill, S. L. L., Lysenko, I., De Palma, A., Phillips, H. R. P., Senior, R. A., Bennett, D. J., Booth, H., Choimes, A., Correia, D. L. P., Day, J., Echeverría‐Londoño, S., Garon, M., Harrison, M. L. K., Ingram, D. J., Jung, M., Kemp, V., … Purvis, A. (2014). The PREDICTS database: A global database of how local terrestrial biodiversity responds to human impacts. Ecology and Evolution, 4(24), 4701–4735. https://doi.org/10.1002/ece3.1303
Hulme, P. E. (2021). Unwelcome exchange: International trade as a direct and indirect driver of biological invasions worldwide. One Earth, 4(5), 666–679. https://doi.org/10.1016/j.oneear.2021.04.015
IPBES. (2023). Summary for policymakers of the thematic assessment report on invasive alien species and their control of the intergovernmental science‐policy platform on biodiversity and ecosystem services. https://doi.org/10.5281/zenodo.7430692
Jandt, U., Bruelheide, H., Jansen, F., Bonn, A., Grescho, V., Klenke, R. A., Sabatini, F. M., Bernhardt‐Römermann, M., Blüml, V., Dengler, J., Diekmann, M., Doerfler, I., Döring, U., Dullinger, S., Haider, S., Heinken, T., Horchler, P., Kuhn, G., Lindner, M., … Wulf, M. (2022). More losses than gains during one century of plant biodiversity change in Germany. Nature, 611(7936), 512–518. https://doi.org/10.1038/s41586‐022‐05320‐w
Kalusová, V., Chytrý, M., Van Kleunen, M., Mucina, L., Dawson, W., Essl, F., Kreft, H., Pergl, J., Weigelt, P., Winter, M., & Pyšek, P. (2017). Naturalization of European plants on other continents: The role of donor habitats. Proceedings of the National Academy of Sciences of the United States of America, 114(52), 13756–13761. https://doi.org/10.1073/pnas.1705487114
Kassambara, A., Kosinski, M., Biecek, P., & Fabian, S. (2021). Package “survminer”.
Kumschick, S., Bacher, S., Evans, T., Marková, Z., Pergl, J., Pyšek, P., Vaes‐Petignat, S., van der Veer, G., Vilà, M., & Nentwig, W. (2015). Comparing impacts of alien plants and animals in Europe using a standard scoring system. Journal of Applied Ecology, 52(3), 552–561. https://doi.org/10.1111/1365‐2664.12427
Latham, A. D. M., Warburton, B., Byrom, A. E., & Pech, R. P. (2017). The ecology and management of mammal invasions in forests. Biological Invasions, 19(11), 3121–3139. https://doi.org/10.1007/s10530‐017‐1421‐5
Lebbink, G., Dwyer, J. M., & Fensham, R. J. (2022). ‘Invasion debt’ after extensive land‐use change: An example from eastern Australia. Journal of Environmental Management, 302, 114051. https://doi.org/10.1016/j.jenvman.2021.114051
Liu, D., Semenchuk, P., Essl, F., Lenzner, B., Moser, D., Blackburn, T. M., Cassey, P., Biancolini, D., Capinha, C., Dawson, W., Dyer, E. E., Guénard, B., Economo, E. P., Kreft, H., Pergl, J., Pyšek, P., van Kleunen, M., Nentwig, W., Rondinini, C., … Dullinger, S. (2023). The impact of land use on non‐native species incidence and number in local assemblages worldwide. Nature Communications, 14(1), 2090. https://doi.org/10.1038/s41467‐023‐37571‐0
Lüdecke, D. (2018). Ggeffects: Tidy data frames of marginal effects from regression models. Journal of Open Source Software, 3(26), 772. https://doi.org/10.21105/joss.00772
MacDougall, A. S., McCann, K. S., Gellner, G., & Turkington, R. (2013). Diversity loss with persistent human disturbance increases vulnerability to ecosystem collapse. Nature, 494(7435), 86–89. https://doi.org/10.1038/nature11869
Newbold, T., Hudson, L. N., Contu, S., Hill, S. L. L., Beck, J., Liu, Y., Meyer, C., Phillips, H. R. P., Scharlemann, J. P. W., & Purvis, A. (2018). Widespread winners and narrow‐ranged losers: Land use homogenizes biodiversity in local assemblages worldwide. PLoS Biology, 16(12), e2006841. https://doi.org/10.1371/journal.pbio.2006841
Newbold, T., Hudson, L. N., Hill, S. L. L., Contu, S., Lysenko, I., Senior, R. A., Börger, L., Bennett, D. J., Choimes, A., Collen, B., Day, J., De Palma, A., Díaz, S., Echeverria‐Londoño, S., Edgar, M. J., Feldman, A., Garon, M., Harrison, M. L. K., Alhusseini, T., … Purvis, A. (2015). Global effects of land use on local terrestrial biodiversity. Nature, 520(7545), 45–50. https://doi.org/10.1038/nature14324
Olson, D. M., Dinerstein, E., Wikramanayake, E. D., Burgess, N. D., Powell, G. V., Underwood, E. C., D'amico, J. A., Itoua, I., Strand, H. E., Morrison, J. C., & Loucks, C. J. (2001). Terrestrial ecoregions of the world: A new map of life on earth: A new global map of terrestrial ecoregions provides an innovative tool for conserving biodiversity. Bioscience, 51(11), 933–938.
Pyšek, P., Hulme, P. E., Simberloff, D., Bacher, S., Blackburn, T. M., Carlton, J. T., Dawson, W., Essl, F., Foxcroft, L. C., Genovesi, P., Jeschke, J. M., Kühn, I., Liebhold, A. M., Mandrak, N. E., Meyerson, L. A., Pauchard, A., Pergl, J., Roy, H. E., Seebens, H., … Richardson, D. M. (2020). Scientists' warning on invasive alien species. Biological Reviews, 95(6), 1511–1534. https://doi.org/10.1111/brv.12627
Pyšek, P., Pergl, J., Essl, F., Lenzner, B., Dawson, W., Kreft, H., Weigelt, P., Winter, M., Kartesz, J., Nishino, M., Antonova, L. A., Barcelona, J. F., Cabezas, F. J., Cárdenas, D., Cárdenas‐Toro, J., Castaño, N., Chacón, E., Chatelain, C., Dullinger, S., … Van Kleunen, M. (2017). Naturalized alien flora of the world: Species diversity, taxonomic and phylogenetic patterns, geographic distribution and global hotspots of plant invasion. Preslia, 89(3), 203–274. https://doi.org/10.23855/preslia.2017.203
R Core Team (2023) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing Vienna. https://www.R‐project.org/
Robeck, P., Essl, F., van Kleunen, M., Pyšek, P., Pergl, J., Weigelt, P., & Mesgaran, M. B. (2024). Invading plants remain undetected in a lag phase while they explore suitable climates. Nature Ecology & Evolution, 8(3), 477–488. https://doi.org/10.1038/s41559‐023‐02313‐4
Rouget, M., Robertson, M. P., Wilson, J. R. U., Hui, C., Essl, F., Renteria, J. L., & Richardson, D. M. (2016). Invasion debt—Quantifying future biological invasions. Diversity and Distributions, 22(4), 445–456. https://doi.org/10.1111/ddi.12408
Seebens, H., Bacher, S., Blackburn, T. M., Capinha, C., Dawson, W., Dullinger, S., Genovesi, P., Hulme, P. E., van Kleunen, M., Kühn, I., Jeschke, J. M., Lenzner, B., Liebhold, A. M., Pattison, Z., Pergl, J., Pyšek, P., Winter, M., & Essl, F. (2021). Projecting the continental accumulation of alien species through to 2050. Global Change Biology, 27(5), 970–982. https://doi.org/10.1111/gcb.15333
Seebens, H., Blackburn, T. M., Dyer, E. E., Genovesi, P., Hulme, P. E., Jeschke, J. M., Pagad, S., Pyšek, P., Winter, M., Arianoutsou, M., Bacher, S., Blasius, B., Brundu, G., Capinha, C., Celesti‐Grapow, L., Dawson, W., Dullinger, S., Fuentes, N., Jäger, H., … Essl, F. (2017). No saturation in the accumulation of alien species worldwide. Nature Communications, 8, 1–9. https://doi.org/10.1038/ncomms14435
Smithson, M., & Verkuilen, J. (2006). A better lemon squeezer? Maximum‐likelihood regression with beta‐distributed dependent variables. Psychological Methods, 11(1), 54. https://doi.org/10.1037/1082‐989x.11.1.54.supp
Tedeschi, L., Biancolini, D., Capinha, C., Rondinini, C., & Essl, F. (2022). Introduction, spread, and impacts of invasive alien mammal species in Europe. Mammal Review, 52(2), 252–266. https://doi.org/10.1111/mam.12277
Therneau, T. (2022). Package “coxme.” mixed effects cox models.
Therneau, T. (2023). A package for survival analysis in R.
van Kleunen, M., Dawson, W., Essl, F., Pergl, J., Winter, M., Weber, E., Kreft, H., Weigelt, P., Kartesz, J., Nishino, M., Antonova, L. A., Barcelona, J. F., Cabezas, F. J., Cárdenas, D., Cárdenas‐Toro, J., Castaño, N., Chacón, E., Chatelain, C., Ebel, A. L., … Pyšek, P. (2015). Global exchange and accumulation of non‐native plants. Nature, 525(7567), 100–103. https://doi.org/10.1038/nature14910
van Kleunen, M., Pyšek, P., Dawson, W., Essl, F., Kreft, H., Pergl, J., Weigelt, P., Stein, A., Dullinger, S., König, C., Lenzner, B., Maurel, N., Moser, D., Seebens, H., Kartesz, J., Nishino, M., Aleksanyan, A., Ansong, M., Antonova, L. A., … Winter, M. (2019). The Global Naturalized Alien Flora (GloNAF) database. Ecology, 100(1), e02542. https://doi.org/10.1002/ecy.2542
Vilà, M., Espinar, J. L., Hejda, M., Hulme, P. E., Jarošík, V., Maron, J. L., Pergl, J., Schaffner, U., Sun, Y., & Pyšek, P. (2011). Ecological impacts of invasive alien plants: A meta‐analysis of their effects on species, communities and ecosystems. Ecology Letters, 14(7), 702–708. https://doi.org/10.1111/j.1461‐0248.2011.01628.x
Wickham, H. (2011). ggplot2. Wiley Interdisciplinary Reviews: Computational Statistics, 3(2), 180–185. https://doi.org/10.1002/wics.147
Williamson, M., Dehnen‐Schmutz, K., Kühn, I., Hill, M., Klotz, S., Milbau, A., Stout, J., & Pyšek, P. (2009). The distribution of range sizes of native and alien plants in four European countries and the effects of residence time. Diversity and Distributions, 15(1), 158–166. https://doi.org/10.1111/j.1472‐4642.2008.00528.x
Wood, S., & Scheipl, F. (2022). Package “gamm4” title generalized additive mixed models using “mgcv” and “lme4.”.
Xu, W.‐B., Blowes, S. A., Brambilla, V., Chow, C. F. Y., Fontrodona‐Eslava, A., Martins, I. S., McGlinn, D., Moyes, F., Sagouis, A., Shimadzu, H., van Klink, R., Magurran, A. E., Gotelli, N. J., McGill, B. J., Dornelas, M., & Chase, J. M. (2023). Regional occupancy increases for widespread species but decreases for narrowly distributed species in metacommunity time series. Nature Communications, 14(1), 1463. https://doi.org/10.1038/s41467‐023‐37127‐2
Yang, Q., Weigelt, P., Fristoe, T. S., Zhang, Z., Kreft, H., Stein, A., Seebens, H., Dawson, W., Essl, F., König, C., Lenzner, B., Pergl, J., Pouteau, R., Pyšek, P., Winter, M., Ebel, A. L., Fuentes, N., Giehl, E. L. H., Kartesz, J., … van Kleunen, M. (2021). The global loss of floristic uniqueness. Nature Communications, 12(1), 7290. https://doi.org/10.1038/s41467‐021‐27603‐y