Antiproliferative effect of low-level laser/ photobiomodulation on gingival fibroblasts derived from calcium channel blocker-induced gingival overgrowth.
Humans
Fibroblasts
/ radiation effects
Low-Level Light Therapy
/ methods
Gingival Overgrowth
/ chemically induced
Calcium Channel Blockers
/ pharmacology
Cell Proliferation
/ radiation effects
Gingiva
/ radiation effects
Connective Tissue Growth Factor
/ metabolism
Cells, Cultured
Collagen Type I
/ metabolism
Transforming Growth Factor beta1
/ metabolism
Cell Survival
/ radiation effects
Lasers, Semiconductor
/ therapeutic use
Male
Adult
Female
CTGF
Calcium channel blockers
Collagen
Gingival enlargement
Low level laser therapy
Photobiomodulation
TGF- β
Journal
Lasers in medical science
ISSN: 1435-604X
Titre abrégé: Lasers Med Sci
Pays: England
ID NLM: 8611515
Informations de publication
Date de publication:
25 Jul 2024
25 Jul 2024
Historique:
received:
01
03
2024
accepted:
24
06
2024
medline:
26
7
2024
pubmed:
26
7
2024
entrez:
25
7
2024
Statut:
epublish
Résumé
The aim of this study was to evaluate the antiproliferative properties of low-level laser therapy (LLLT) on gingival fibroblasts obtained from calcium channel blocker-induced gingival overgrowth (GO). Gingival fibroblasts of patients with GO were compared to healthy gingival fibroblasts (H). Both cells were exposed to LLLT (685 nm wavelength, 25mW power, diode laser) and compared to those not treated with LLLT. Cell proliferation and viability were measured with MTT assay at baseline and after 24 and 72 h. TGF-β1, CTGF, and collagen Type 1 levels were evaluated with Enzyme-Linked Immunosorbent Assay (ELISA). LLLT significantly decreased the proliferation of GO fibroblasts (p < 0.05) while leading to a significantly higher proliferation in H fibroblasts compared to the untreated cells (p < 0.05). GO cells showed significantly higher CTGF, TGF-β, and collagen Type 1 expression than the H cells (p < 0.05). LLLT significantly reduced CTGF levels in GO cells compared to the control group (p < 0.05). In H cells, CTGF and TGF-β levels were also significantly decreased in response to LLLT compared to the control group (p < 0.05). While LLLT significantly reduced collagen expression in the H group (p < 0.05), it did not significantly impact the GO cells. LLLT significantly reduced the synthesis of the growth factors and collagen in both groups with an antiproliferative effect on the gingival fibroblasts from calcium channel blocker-induced GO, suggesting that it can offer a therapeutic approach in the clinical management of drug-induced GO, reversing the fibrotic changes.
Identifiants
pubmed: 39052077
doi: 10.1007/s10103-024-04122-y
pii: 10.1007/s10103-024-04122-y
doi:
Substances chimiques
Calcium Channel Blockers
0
Connective Tissue Growth Factor
139568-91-5
Collagen Type I
0
Transforming Growth Factor beta1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
194Informations de copyright
© 2024. The Author(s).
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