Indoor residual spraying of experimental huts in Cameroon highlights the potential of Fludora® Fusion to control wild pyrethroid-resistant malaria vectors.
Animals
Pyrethrins
/ pharmacology
Anopheles
/ drug effects
Insecticide Resistance
/ genetics
Insecticides
/ pharmacology
Mosquito Control
/ methods
Cameroon
Mosquito Vectors
/ drug effects
Malaria
/ transmission
Guanidines
/ pharmacology
Nitriles
/ pharmacology
Female
Thiazoles
/ pharmacology
Neonicotinoids
/ pharmacology
Housing
GSTe2
Kdr
Anopheles
Clothianidin
Fludora® Fusion
Indoor residual spraying
Insecticide resistance
Malaria
Vector control
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
25 Jul 2024
25 Jul 2024
Historique:
received:
25
01
2024
accepted:
17
07
2024
medline:
26
7
2024
pubmed:
26
7
2024
entrez:
25
7
2024
Statut:
epublish
Résumé
Elevated resistance to pyrethroids in major malaria vectors has led to the introduction of novel insecticides including neonicotinoids. There is a fear that efficacy of these new insecticides could be impacted by cross-resistance mechanisms from metabolic resistance to pyrethroids. In this study, after evaluating the resistance to deltamethrin, clothianidin and mixture of clothianidin + deltamethrin in the lab using CDC bottle assays, the efficacy of the new IRS formulation Fludora® Fusion was tested in comparison to clothianidin and deltamethrin applied alone using experimental hut trials against wild free-flying pyrethroid-resistant Anopheles funestus from Elende and field An. gambiae collected from Nkolondom reared in the lab and released in the huts. Additionally, cone tests on the treated walls were performed each month for a period of twelve months to evaluate the residual efficacy of the sprayed products. Furthermore, the L1014F-kdr target-site mutation and the L119F-GSTe2 mediated metabolic resistance to pyrethroids were genotyped on a subset of mosquitoes from the EHT to assess the potential cross-resistance. All Anopheles species tested were fully susceptible to clothianidin and clothianidin + deltamethrin mixture in CDC bottle assay while resistance was noted to deltamethrin. Accordingly, Fludora® Fusion (62.83% vs 42.42%) and clothianidin (64.42% vs 42.42%) induced significantly higher mortality rates in EHT than deltamethrin (42.42%) against free flying An. funestus from Elende in month 1 (M1) and no significant difference in mortality was observed between the first (M1) and sixth (M6) months of the evaluation (P > 0.05). However, lower mortality rates were recorded against An. gambiae s.s from Nkolondom (mortality rates 50%, 45.56% and 26.68%). In-situ cone test on the wall showed a high residual efficacy of Fludora® Fusion and clothianidin on the susceptible strain KISUMU (> 12 months) and moderately on the highly pyrethroid-resistant An. gambiae strain from Nkolondom (6 months). Interestingly, no association was observed between the L119F-GSTe2 mutation and the ability of mosquitoes to survive exposure to Fludora® Fusion, whereas a trend was observed with the L1014F-kdr mutation. This study highlights that Fludora® Fusion, through its clothianidin component, has good potential of controlling pyrethroid-resistant mosquitoes with prolonged residual efficacy. This could be therefore an appropriate tool for vector control in several malaria endemic regions.
Identifiants
pubmed: 39054424
doi: 10.1186/s12879-024-09630-4
pii: 10.1186/s12879-024-09630-4
doi:
Substances chimiques
Pyrethrins
0
Insecticides
0
Guanidines
0
Nitriles
0
clothianidin
2V9906ABKQ
decamethrin
2JTS8R821G
Thiazoles
0
Neonicotinoids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
733Subventions
Organisme : Wellcome Trust Senior Research Fellowship
ID : 217188/Z/19/Z
Organisme : Bill & Melinda Gates Foundation
ID : INV-006003
Pays : United States
Informations de copyright
© 2024. The Author(s).
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