Determination of nutrient concentration in liquid culture of cyanobacteria Nostoc sp. by capillary electrophoresis and inductively coupled plasma mass spectrometry.

capillary electrophoresis cyanobacteria nutrient uptake photobioreactor

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
24 Jul 2024
Historique:
received: 20 12 2023
revised: 27 06 2024
accepted: 23 07 2024
medline: 27 7 2024
pubmed: 27 7 2024
entrez: 26 7 2024
Statut: aheadofprint

Résumé

In this work, we demonstrate the use of capillary electrophoresis and inductively coupled plasma mass spectrometry, as competitive methods primarily for ion chromatography, to determine changes in the concentration of small inorganic ions in the Nostoc sp. culture medium. Although macronutrients were analyzed by capillary electrophoresis with conductivity detection, micronutrients were analyzed by inductively coupled plasma mass spectrometry. The different light settings (light intensity and spectral composition) had a visible effect on the culture growth and depletion of calcium, magnesium, and phosphate ions, and iron and manganese elements when comparing the behavior under red or violet light with that under blue light.

Identifiants

pubmed: 39059566
pii: S0003-2697(24)00174-X
doi: 10.1016/j.ab.2024.115630
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

115630

Informations de copyright

Copyright © 2024. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. ☐ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:

Auteurs

Natália Melicherová (N)

Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 967/97, 602 00 Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic.

Tomáš Vaculovič (T)

Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic; Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina, Ilkovičova 6, 842 15 Bratislava, Slovakia.

Radka Kočí (R)

PSI (Photon Systems Instruments), spol. s r.o., Průmyslová 470, 664 24, Drásov, Czech Republic.

Martin Trtílek (M)

PSI (Photon Systems Instruments), spol. s r.o., Průmyslová 470, 664 24, Drásov, Czech Republic.

Jana Lavická (J)

Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 967/97, 602 00 Brno, Czech Republic. Electronic address: lavicka@iach.cz.

František Foret (F)

Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 967/97, 602 00 Brno, Czech Republic.

Classifications MeSH