Surra-affected dromedary camels show reduced numbers of blood B-cells and in vitro evidence of Trypanosoma-induced B cell death.
Apoptosis
B cells
Camel
Flow cytometry
Surra
Trypanosomosis
Journal
Tropical animal health and production
ISSN: 1573-7438
Titre abrégé: Trop Anim Health Prod
Pays: United States
ID NLM: 1277355
Informations de publication
Date de publication:
26 Jul 2024
26 Jul 2024
Historique:
received:
22
03
2024
accepted:
18
07
2024
medline:
27
7
2024
pubmed:
27
7
2024
entrez:
26
7
2024
Statut:
epublish
Résumé
Trypanosomosis due to Trypanosoma evansi (surra) is one of the most important diseases with a significant impact on camel health and production. Trypanosoma-induced immunosuppression mechanisms, which are key factors of disease pathogenesis, have been characterized in several animal species. The present study investigated, therefore, the impact of trypanosomosis on the immunophenotype of blood leukocytes in camels. For this, the relative and absolute values of blood leukocyte populations, their expression pattern of cell surface molecules, and the numbers of the main lymphocyte subsets were compared between healthy camels and camels with clinical symptoms of chronic surra and serological evidence of exposure to Trypanosoma infection. Leukocytes were separated from the blood of healthy and diseased camels, labeled with fluorochrome-conjugated antibodies, and analyzed by flow cytometry. Compared to healthy camels, the leukogram of diseased camels was characterized by a slightly increased leukocyte count with moderate neutrophilia and monocytosis indicating a chronic inflammatory pattern that may reflect tissue injury due to the long-lasting inflammation. In addition, the analysis of lymphocyte subsets revealed a lower number and percentage of B cells in diseased than healthy camels. In vitro incubation of camel mononuclear cells with fluorochrome-labeled T. evansi revealed a higher capacity of camel B cells than T cells to bind the parasite in vitro. Furthermore, cell viability analysis of camel PBMC incubated in vitro with T. evansi whole parasites but not the purified antigens resulted in Trypanosoma-induced apoptosis and necrosis of camel B cells. Here we demonstrate an association between trypanosomosis in camels and reduced numbers of blood B cells. In vitro analysis supports a high potential of T. evansi to bind to camel B cells and induce their elimination by apoptosis and necrosis.
Identifiants
pubmed: 39060802
doi: 10.1007/s11250-024-04078-9
pii: 10.1007/s11250-024-04078-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
223Subventions
Organisme : Deanship of Scientific Research, Princess Nourah Bint Abdulrahman University
ID : PNURSP2024R460
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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