Location Is Everything: Influence of His-Tag Fusion Site on Properties of Adenylosuccinate Synthetase from
Helicobacter pylori
His-tag
adenylosuccinate synthetase
enzyme kinetics
protein stability
protein structure
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
11 Jul 2024
11 Jul 2024
Historique:
received:
30
04
2024
revised:
05
07
2024
accepted:
08
07
2024
medline:
27
7
2024
pubmed:
27
7
2024
entrez:
27
7
2024
Statut:
epublish
Résumé
The requirement for fast and dependable protein purification methods is constant, either for functional studies of natural proteins or for the production of biotechnological protein products. The original procedure has to be formulated for each individual protein, and this demanding task was significantly simplified by the introduction of affinity tags.
Identifiants
pubmed: 39062851
pii: ijms25147613
doi: 10.3390/ijms25147613
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Croatian Science Foundation
ID : IP-2013-11-7423
Organisme : Croatian Science Foundation
ID : IP-2019-04-6764
Organisme : National Science Center
ID : Harmonia 2015/18/M/NZ1/00776
Organisme : University of Warsaw
ID : IDUB PSP-501-D111-20-0004316
Organisme : Polish Ministry for Science and Higher Education
ID : 501-D111-01-1110102
Organisme : ERDF
ID : POIG.02.02.00-00-025/09
Organisme : ERDF
ID : POIG.02.01.00-14-122/09