Prevalence of Mycobacterium leprae and Mycobacterium lepromatosis in roadkill armadillos in Brazil.

To evaluate the prevalence of Mycobacterium leprae and Mycobacterium lepromatosis in road killed armadillos identified along Brazilian regions, samples of liver, spleen, muscle, ear, nose and tail were collected on highways from 78 animals. The armadillos were of four different species, Cabassous tatouay, Dasypus novemcinctus, Dasypus septemcinctus and Euphractus sexcinctus. After DNA extraction from two tissues, specific primers were used for the detection of each pathogen using SYBR green qualitative Real-Time PCR, and amplicons were sequenced. The species with the highest prevalence was Dasypus novemcinctus, mainly in the Central-West, South, and Southeast regions of Brazil. We detected M. leprae DNA in 32 (41%) of the 78 individuals and M. lepromatosis DNA was not identified in any of the examined samples. The zoonotic component of leprosy may play a role in the transmission of the disease in endemic areas in which environmental conditions and contact with reservoirs must be investigated.

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Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The author is an Editorial Board Member/Editor-in-Chief/Associate Editor/Guest Editor for [ActaTropica] and was not involved in the editorial review or the decision to publish this article. The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Financial support was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code: CAPES Epidemias 0670/2020, Number 88881.505199/2020-01, Brasília, Brazil. Postdoctoral Scholarship by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) 88887.671152/2022-00 (Lara, JM). Postdoctoral Scholarship by The São Paulo Research Foundation (FAPESP) 2018/12565-6 (Drummond, MR); Ph.D. scholarship by The Brazilian National Council for Scientific and Technological Development (CNPq) 142046/2019-1 (Silva, DP); Productivity Grant by The Brazilian National Council for Scientific and Technological Development (CNPq) 306970/2018-0 (Velho, PENF) and 305778/2021-8 (Donalisio, MR).