Prospective One-Health investigation into low-abundant extended-spectrum β-lactamase producing Enterobacterales enables detection of potential dissemination events and persistence.

Antimicrobial resistance gene reservoirs Clinical and environmental samples ESBL Foodstuffs Incompatibility group genes KESC group Plasmid-replicons Wastewater

Journal

The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500

Informations de publication

Date de publication:
26 Jul 2024
Historique:
received: 05 04 2024
revised: 18 07 2024
accepted: 25 07 2024
medline: 29 7 2024
pubmed: 29 7 2024
entrez: 28 7 2024
Statut: aheadofprint

Résumé

Following a one-health approach, we sought to determine reservoirs of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales (ESBL-PE), other than Escherichia coli or Klebsiella pneumoniae complex species (i.e., low-abundant species), and their associated ESBL genes and plasmid-replicon profiles. From 06/2017-05/2019, ESBL-PE isolates were recovered from clinical samples routinely collected at the University Hospital Basel (Basel, Switzerland), as well as from wastewater and foodstuffs collected monthly at predefined locations throughout the city of Basel. Whole-genome sequencing was performed for characterization of ESBL-PE isolates. Among 1634 isolates recovered, 114 (7 %) belonged to 17 low-abundant ESBL-PE species. Seven species originated from more than one compartment, mainly from clinical and wastewater samples (6/17). Sixteen different ESBL genes were identified, with bla The dominance of bla

Sections du résumé

BACKGROUND BACKGROUND
Following a one-health approach, we sought to determine reservoirs of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales (ESBL-PE), other than Escherichia coli or Klebsiella pneumoniae complex species (i.e., low-abundant species), and their associated ESBL genes and plasmid-replicon profiles.
METHODS METHODS
From 06/2017-05/2019, ESBL-PE isolates were recovered from clinical samples routinely collected at the University Hospital Basel (Basel, Switzerland), as well as from wastewater and foodstuffs collected monthly at predefined locations throughout the city of Basel. Whole-genome sequencing was performed for characterization of ESBL-PE isolates.
RESULTS RESULTS
Among 1634 isolates recovered, 114 (7 %) belonged to 17 low-abundant ESBL-PE species. Seven species originated from more than one compartment, mainly from clinical and wastewater samples (6/17). Sixteen different ESBL genes were identified, with bla
CONCLUSIONS CONCLUSIONS
The dominance of bla

Identifiants

DOI: 10.1016/j.scitotenv.2024.175078 PMID: 39069185
pubmed: 39069185
pii: S0048-9697(24)05228-8
doi: 10.1016/j.scitotenv.2024.175078
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

175078

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Auteurs

Ana B García-Martín (AB)

Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland; Department of Clinical Research, University Hospital Basel, Basel, Switzerland. Electronic address: anabelen.garciamartin@usb.ch.

Lisandra Aguilar-Bultet (L)

Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland; Department of Clinical Research, University Hospital Basel, Basel, Switzerland. Electronic address: lisandra.aguilarbultet@usb.ch.

Elena Gómez-Sanz (E)

Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland; Department of Clinical Research, University Hospital Basel, Basel, Switzerland. Electronic address: elena.gomezsanz@ubs.ch.

Monica Alt Hug (MA)

State Laboratory Basel-City, Basel, Switzerland. Electronic address: monica.alt@bs.ch.

Reto Furger (R)

State Laboratory Basel-City, Basel, Switzerland. Electronic address: r.furger@unibas.ch.

Lucas Eichenberger (L)

State Laboratory Basel-City, Basel, Switzerland. Electronic address: lucas.eichenberger@mibellegroup.com.

Ruth Schindler (R)

Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland; Department of Clinical Research, University Hospital Basel, Basel, Switzerland. Electronic address: ruth.schindler@usb.ch.

Ingrid Steffen (I)

Rothen Laboratory, Basel, Switzerland. Electronic address: i.steffen@labor-rothen.ch.

Adrian Egli (A)

Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address: aegli@imm.uzh.ch.

Tanja Stadler (T)

Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland. Electronic address: tanja.stadler@bsse.ethz.ch.

Claudia Bagutti (C)

State Laboratory Basel-City, Basel, Switzerland. Electronic address: claudia.bagutti@bs.ch.

Sarah Tschudin-Sutter (S)

Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland; Department of Clinical Research, University Hospital Basel, Basel, Switzerland. Electronic address: sarah.tschudin@usb.ch.

Classifications MeSH