Systems genetic analysis of lignin biosynthesis in Populus tremula.
GWAS
HD‐Zip III
Populus
aspen
eQTL
lignin biosynthesis
wood formation
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
28 Jul 2024
28 Jul 2024
Historique:
received:
28
01
2024
accepted:
02
07
2024
medline:
29
7
2024
pubmed:
29
7
2024
entrez:
29
7
2024
Statut:
aheadofprint
Résumé
The genetic control underlying natural variation in lignin content and composition in trees is not fully understood. We performed a systems genetic analysis to uncover the genetic regulation of lignin biosynthesis in a natural 'SwAsp' population of aspen (Populus tremula) trees. We analyzed gene expression by RNA sequencing (RNA-seq) in differentiating xylem tissues, and lignin content and composition using Pyrolysis-GC-MS in mature wood of 268 trees from 99 genotypes. Abundant variation was observed for lignin content and composition, and genome-wide association study identified proteins in the pentose phosphate pathway and arabinogalactan protein glycosylation among the top-ranked genes that are associated with these traits. Variation in gene expression and the associated genetic polymorphism was revealed through the identification of 312 705 local and 292 003 distant expression quantitative trait loci (eQTL). A co-expression network analysis suggested modularization of lignin biosynthesis and novel functions for the lignin-biosynthetic CINNAMYL ALCOHOL DEHYDROGENASE 2 and CAFFEOYL-CoA O-METHYLTRANSFERASE 3. PHENYLALANINE AMMONIA LYASE 3 was co-expressed with HOMEOBOX PROTEIN 5 (HB5), and the role of HB5 in stimulating lignification was demonstrated in transgenic trees. The systems genetic approach allowed linking natural variation in lignin biosynthesis to trees´ responses to external cues such as mechanical stimulus and nutrient availability.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fundação para a Ciência e Tecnologia
ID : CEEC/IND/00175/2017
Organisme : Fundação para a Ciência e Tecnologia
ID : UIDB/04551/2020
Organisme : Fundação para a Ciência e Tecnologia
ID : UIDP/04551/2020
Organisme : Knut och Alice Wallenbergs Stiftelse
ID : 2016.0341
Organisme : Knut och Alice Wallenbergs Stiftelse
ID : 2016.0352
Organisme : VINNOVA
ID : 2016-00504
Organisme : Svenska Forskningsrådet Formas
ID : 2018-01381
Organisme : Svenska Forskningsrådet Formas
ID : 2018-01611
Informations de copyright
© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
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