Combined Flow-Fluorescence in situ hybridization to HHV-8 and EBV reveals the viral heterogeneity of primary effusion lymphoma.
EBV
Flow‐Fluorescence in situ hybridization
HHV8
KSHV
primary effusion lymphoma
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
Aug 2024
Aug 2024
Historique:
revised:
02
07
2024
received:
12
05
2024
accepted:
17
07
2024
medline:
30
7
2024
pubmed:
30
7
2024
entrez:
30
7
2024
Statut:
ppublish
Résumé
Primary effusion lymphoma (PEL) is a rare B-cell non-Hodgkin lymphoma associated with Kaposi Sarcoma-associated herpesvirus (KSHV/HHV8) infection. Lymphoma cells are coinfected with Epstein-Barr virus (EBV) in 60-80% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports PEL diagnosis in 4 patients using a Flow-Fluorescence in situ hybridization (FlowFISH) technique that allowed detection of differentially expressed EBV and HHV8 transcripts within the same sample, revealing viral heterogeneity of the disease. Moreover, infected cells exhibited variable expressions of CD19, CD38, CD40, and CD138. Therefore, FlowFISH is a promising tool to diagnose and characterize complex viral lymphoproliferations.
Types de publication
Journal Article
Case Reports
Langues
eng
Sous-ensembles de citation
IM
Pagination
e29836Informations de copyright
© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.
Références
Simonelli C, Spina M, Cinelli R, et al. Clinical features and outcome of primary effusion lymphoma in HIV‐infected patients: a single‐institution study. J Clin Oncol. 2003;21(21):3948‐3954.
Guillet S, Gérard L, Meignin V, et al. Classic and extracavitary primary effusion lymphoma in 51HIV‐infected patients from a single institution. Am J Hematol. 2016;91(2):233‐237.
Lurain K, Ramaswami R, Marshall V, et al. Kaposi sarcoma herpesvirus viral load as a biomarker for leptomeningeal involvement by primary effusion lymphoma. Haematologica. 2022;108(7):1940‐1944.
Kabiawu‐Ajise OE, Harris J, Ismaili N, Amorosi E, Ibrahim S. Primary effusion lymphoma with central nervous system involvement in an HIV‐negative homosexual male. Acta Haematol. 2012;128(2):77‐82.
Horenstein MG, Nador RG, Chadburn A, et al. Epstein‐Barr virus latent gene expression in primary effusion lymphomas containing Kaposi's sarcoma—associated Herpesvirus/Human Herpesvirus‐8. 1997.
Nador R, Cesarman E, Chadburn A, et al. Primary effusion lymphoma: a distinct clinicopathologic entity associated with the Kaposi's sarcoma‐associated herpes virus. Blood. 1996;88(2):645‐656.
Calvani J, Gérard L, Fadlallah J, et al. A comprehensive clinicopathologic and molecular study of 19 primary effusion lymphomas in HIV‐infected patients. Am J Surg Pathol. 2022;46(3):353‐362.
Lurain K, Polizzotto MN, Aleman K, et al. Viral, immunologic, and clinical features of primary effusion lymphoma. Blood. 2019;133(16):1753‐1761.
Zhou T, Yuan CM, Lurain K, et al. A novel approach for characterization of KSHV‐associated multicentric Castleman disease from effusions. Br J Haematol. 2023;200(4):462‐475.
Marcelin AG, Motol J, Guihot A, et al. Relationship between the quantity of kaposi sarcoma–associated herpesvirus (KSHV) in peripheral blood and effusion fluid samples and KSHV‐associated disease. J Infect Dis. 2007;196(8):1163‐1166.
Fournier B, Boutboul D, Bruneau J, et al. Rapid identification and characterization of infected cells in blood during chronic active Epstein‐Barr virus infection. J Exp Med. 2020;217(11):e20192262.
Martin De Frémont G, Vanjak A, Sbihi Z, et al. Characteristics of circulating KSHV‐infected viroblasts during active KSHV+ multicentric Castleman disease. Blood Adv. 2023;7(9):1682‐1691.