An ancient role for CYP73 monooxygenases in phenylpropanoid biosynthesis and embryophyte development.
Biopolymers
Bryophytes
Cinnamic Acid 4-Hydroxylase
Cytochromes P450
Plant Evolution
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
01 Aug 2024
01 Aug 2024
Historique:
received:
29
10
2023
accepted:
15
07
2024
revised:
03
07
2024
medline:
2
8
2024
pubmed:
2
8
2024
entrez:
1
8
2024
Statut:
aheadofprint
Résumé
The phenylpropanoid pathway is one of the plant metabolic pathways most prominently linked to the transition to terrestrial life, but its evolution and early functions remain elusive. Here, we show that activity of the t-cinnamic acid 4-hydroxylase (C4H), the first plant-specific step in the pathway, emerged concomitantly with the CYP73 gene family in a common ancestor of embryophytes. Through structural studies, we identify conserved CYP73 residues, including a crucial arginine, that have supported C4H activity since the early stages of its evolution. We further demonstrate that impairing C4H function via CYP73 gene inactivation or inhibitor treatment in three bryophyte species-the moss Physcomitrium patens, the liverwort Marchantia polymorpha and the hornwort Anthoceros agrestis-consistently resulted in a shortage of phenylpropanoids and abnormal plant development. The latter could be rescued in the moss by exogenous supply of p-coumaric acid, the product of C4H. Our findings establish the emergence of the CYP73 gene family as a foundational event in the development of the plant phenylpropanoid pathway, and underscore the deep-rooted function of the C4H enzyme in embryophyte biology.
Identifiants
pubmed: 39090438
doi: 10.1038/s44318-024-00181-7
pii: 10.1038/s44318-024-00181-7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-10-IDEX-0002-02
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-19-CE20-0017
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : EXC-2189,CIBSS
Organisme : Uni Freiburg | Freiburg Institute for Advanced Studies, Albert-Ludwigs-Universität Freiburg (FRIAS)
ID : METABEVO
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : Overseas Research Fellowships
Organisme : Conseil régional du Grand Est (Conseil Régional Grand Est)
ID : Vitest
Informations de copyright
© 2024. The Author(s).
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