Phage probe on RAFT polymer surface for rapid enumeration of E. coli K12.

Bacteriophage Impedimetric detection Pathogenic bacteria Polymeric film

Journal

Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583

Informations de publication

Date de publication:
29 Jul 2024
Historique:
received: 09 03 2024
revised: 24 07 2024
accepted: 26 07 2024
medline: 3 8 2024
pubmed: 3 8 2024
entrez: 2 8 2024
Statut: aheadofprint

Résumé

This study presents a simple, fast, and sensitive label-free sensing assay for the precise enumeration of modeled pathogenic Escherichia coli K12 (E. coli K12) bacteria for the first time. The method employs the covalent binding bacteriophage technique on the surface of a reversible addition-fragmentation chain transfer (RAFT) polymer film. The Nyquist plots obtained from electrochemical impedance spectroscopy (EIS) identified the charge transfer resistance R

Identifiants

DOI: 10.1016/j.bioelechem.2024.108785 PMID: 39094446
pubmed: 39094446
pii: S1567-5394(24)00147-6
doi: 10.1016/j.bioelechem.2024.108785
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

108785

Informations de copyright

Copyright © 2024 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Sallahuddin Panhwar (S)

Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330 Ankara, Türkiye; Department of Civil Engineering, National University of Sciences and Technology, Quetta 24090, Balochistan, Pakistan.

Hüseyin Çelikkan (H)

Gazi University, Faculty of Science, Department of Chemistry, Ankara, Türkiye.

Eylul Evran (E)

Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe 06800 Ankara, Türkiye.

Esra Ekiz (E)

Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe 06800 Ankara, Türkiye.

Kubra Ozkan Hukum (K)

Gazi University, Faculty of Science, Department of Chemistry, Ankara, Türkiye.

Demet Çetin (D)

Department of Mathematics and Science Education, Gazi Faculty of Education, Gazi University, Besevler, Ankara 06500, Türkiye.

Zekiye Suludere (Z)

Faculty of Science, Department of Biology, Gazi University, 06500 Ankara, Türkiye.

Ismail Hakki Boyaci (I)

Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe 06800 Ankara, Türkiye.

Uğur Tamer (U)

Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330 Ankara, Türkiye; METU MEMS Research and Application Center, Ankara, Türkiye. Electronic address: utamer@gazi.edu.tr.

Classifications MeSH