Pokemon inhibits Bim transcription to promote the proliferation, anti-anoikis, invasion, histological grade, and dukes stage of colorectal neoplasms.
Humans
Colorectal Neoplasms
/ pathology
Bcl-2-Like Protein 11
/ genetics
Cell Proliferation
Female
Male
Transcription Factors
/ genetics
Middle Aged
Neoplasm Invasiveness
Anoikis
/ genetics
DNA-Binding Proteins
/ genetics
Neoplasm Staging
Aged
Neoplasm Grading
Gene Expression Regulation, Neoplastic
Transcription, Genetic
Apoptosis
Cell Line, Tumor
Anoikis
Bim
Colorectal neoplasms
Pokemon
Journal
Journal of cancer research and clinical oncology
ISSN: 1432-1335
Titre abrégé: J Cancer Res Clin Oncol
Pays: Germany
ID NLM: 7902060
Informations de publication
Date de publication:
03 Aug 2024
03 Aug 2024
Historique:
received:
14
05
2024
accepted:
20
07
2024
medline:
3
8
2024
pubmed:
3
8
2024
entrez:
2
8
2024
Statut:
epublish
Résumé
This study aims to determine whether Pokemon regulates Bim activity in colorectal carcinoma (CRC) carcinogenesis. Clinical tissue samples were analyzed to detect the expression and clinicopathological significance of Pokemon and Bim in CRC. Proliferation, apoptosis, and invasion assays were conducted to identify the regulatory effect of Pokemon on Bim. The combined treatment effects of Pokemon knockdown and diamminedichloroplatinum (DDP) were also examined. Immunohistochemical analysis of 80 samples of colorectal epithelia (CRE), 80 cases of colorectal adenoma (CRA), and 160 of CRC samples revealed protein expression rates of 23.8%, 38.8%, and 70.6% for Pokemon, and 88.8%, 73.8%, and 31.9% for Bim, respectively. A significant negative correlation was observed between Pokemon and Bim expression across the CRE, CRA, and CRC lesion stages. In CRC, higher Pokemon and lower Bim expression correlated with higher histological grades, advanced Dukes stages, and increased cancer invasion. In both LoVo and HCT116 cells, overexpression of Pokemon significantly reduced Bim expression, leading to increased proliferation, resistance to anoikis, and cell invasion. Additionally, Pokemon overexpression significantly decreased DDP-induced Bim expression, reduction of anti-apoptosis and invasion, whereas Pokemon knockdown resulted in the opposite effects. These findings suggest that Pokemon inhibits Bim transcription, thereby promoting CRC proliferation, resistance to apoptosis, invasion, and advancing histological grade and Dukes staging. Pokemon knockdown enhances the therapeutic efficacy of DDP in the treatment of CRC.
Identifiants
pubmed: 39095579
doi: 10.1007/s00432-024-05904-1
pii: 10.1007/s00432-024-05904-1
doi:
Substances chimiques
Bcl-2-Like Protein 11
0
ZBTB7A protein, human
0
Transcription Factors
0
DNA-Binding Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
380Subventions
Organisme : The Natural Science Foundation of Guangdong Province
ID : 2015A030310046
Organisme : The Natural Science Foundation of Guangdong Province
ID : 2016A030313674
Organisme : Chinese Medicine Research Foundation of Guangdong Province
ID : 20161141
Organisme : Science and Technology Planning Project of Dongguan City
ID : 2016108101028
Organisme : Research Foundation Project of Guangdong Medical University
ID : Z2015004
Organisme : Research Foundation Project of Guangdong Medical University
ID : Z2017005
Organisme : Science and Technology Planning Project of Guangdong Province
ID : 2016A020215224
Informations de copyright
© 2024. The Author(s).
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