Loop-Mediated Isothermal Amplification for the Diagnosis of Sporotrichosis by Sporothrix brasiliensis.
Sporothrix spp
LAMP
Molecular assay
Zoonosis
Journal
Mycopathologia
ISSN: 1573-0832
Titre abrégé: Mycopathologia
Pays: Netherlands
ID NLM: 7505689
Informations de publication
Date de publication:
03 Aug 2024
03 Aug 2024
Historique:
received:
11
11
2023
accepted:
25
07
2024
medline:
4
8
2024
pubmed:
4
8
2024
entrez:
3
8
2024
Statut:
epublish
Résumé
We aimed to develop and validate a Loop-mediated Isothermal Amplification (LAMP) assay to Sporothrix brasiliensis. LAMP reaction was developed using six primers designed based on calmodulin gene. In the LAMP reaction, we tested twenty isolates of S. brasiliensis from animals and humans, along with ten tissue samples extracted from the left footpad of mice that had been experimentally infected with S. brasiliensis. In addition, it included DNA samples from various other fungal species for specificity evaluation. All S. brasiliensis isolates yielded positive results in the LAMP, and the limit of DNA detection was 1 ng/μL. All murine samples were positive in the test while DNA from other fungal species were all negative, resulting in 100% of sensitivity and specificity of primers. LAMP diagnosis technique is a promising alternative to sporotrichosis diagnosis, in a simple and cost-effective way. Further studies are warranted to validate this technique using animal model samples obtained from both humans and animals.
Identifiants
pubmed: 39096410
doi: 10.1007/s11046-024-00880-z
pii: 10.1007/s11046-024-00880-z
doi:
Substances chimiques
DNA Primers
0
Calmodulin
0
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
73Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 405653/2021-2
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 308315/2021-9
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 316067/2021-0
Organisme : Programa Inova Fiocruz
ID : PRES-008-FIO-22-2-11
Organisme : Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
ID : E-26/201.441/2021
Organisme : Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
ID : E-26/200.381/2023
Organisme : Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
ID : 23/2551-0000503-4
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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