Freezing does not influence the microarchitectural parameters of the microstructure of the freshly harvested femoral head bone.
Conservation
Freshly harvested femoral head
Micro-computed tomography
Microarchitecture
Microstructure
Trabecular bone
Journal
Cell and tissue banking
ISSN: 1573-6814
Titre abrégé: Cell Tissue Bank
Pays: Netherlands
ID NLM: 100965121
Informations de publication
Date de publication:
05 Aug 2024
05 Aug 2024
Historique:
received:
21
06
2024
accepted:
17
07
2024
medline:
6
8
2024
pubmed:
6
8
2024
entrez:
5
8
2024
Statut:
aheadofprint
Résumé
The femoral head is one of the most commonly used bones for allografts and biomechanical studies. However, there are few reports on the trabecular bone microarchitectural parameters of freshly harvested trabecular bones. To our knowledge, this is the first study to characterize the microstructure of femoral heads tested immediately after surgery and compare it with the microstructure obtained with conventional freezing. This study aims to investigate whether freezing at -80 °C for 6 weeks affects the trabecular microstructure of freshly harvested bone tissue. This study was divided into two groups: one with freshly harvested human femoral heads and the other with the same human femoral heads frozen at -80 °C for 6 weeks. Each femoral head was scanned using an X-ray microcomputed tomography scanner (µCT) to obtain the microarchitectural parameters, including the bone volume fraction (BV/TV), the mean trabecular thickness (Tb.th), the trabecular separation (Tb.sp), the degree of anisotropy (DA), and the connectivity density (Conn.D). There was no statistically significant difference between the fresh and the frozen groups for any of the parameters measured. This study shows that freezing at -80 °C for 6 weeks does not alter bone microstructure compared with freshly harvested femoral heads tested immediately after surgery.
Identifiants
pubmed: 39103569
doi: 10.1007/s10561-024-10147-y
pii: 10.1007/s10561-024-10147-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Aspenberg P, Thorén K (1990) Lipid extraction enhances bank bone incorporation: an experiment in rabbits. Acta Orthop Scand 61(6):546–548. https://doi.org/10.3109/17453679008993579
doi: 10.3109/17453679008993579
pubmed: 2281762
Beaupied H, Dupuis A, Arlettaz A, Brunet-Imbault B, Bonnet N, Jaffré C, Benhamou C-L, Courteix D (2006) The mode of bone conservation does not affect the architecture and the tensile properties of rat femurs. Bio-Med Mater Eng 16(4):253–259
Beer AJ, Tauro TM, Redondo ML, Christian DR, Cole BJ, Frank RM (2019) Use of allografts in orthopaedic surgery: safety, procurement, storage, and outcomes. Orthop J Sports Med 7(12):2325967119891435. https://doi.org/10.1177/2325967119891435
doi: 10.1177/2325967119891435
pubmed: 31909057
pmcid: 6937533
Birnbaum K, Sindelar R, Gärtner JR, Wirtz DC (2002) Material properties of trabecular bone structures. Surg Radiol Anat 23(6):399–407. https://doi.org/10.1007/s00276-001-0399-x
doi: 10.1007/s00276-001-0399-x
Board TN, Brunskill S, Doree C, Hyde C, Kay PR, Dominic Meek RM, Webster R, Galea G (2009) Processed versus fresh frozen bone for impaction bone grafting in revision hip arthroplasty. Cochrane Database Syst Rev. https://doi.org/10.1002/14651858.CD006351.pub2
doi: 10.1002/14651858.CD006351.pub2
pubmed: 19821362
pmcid: 7386794
Buccino F, Colombo C, Duarte DHL, Rinaudo L, Ulivieri FM, Vergani LM (2021) 2D and 3D numerical models to evaluate trabecular bone damage. Med Biol Eng Compu 59:2139–2152. https://doi.org/10.1007/s11517-021-02422-x
doi: 10.1007/s11517-021-02422-x
Calatayud M, Martín L-O, Librizzi MS, Pablos DL, Méndez VG, Ramos MA, Diaz-Guerra GM, Hawkins F (2021) Trabecular bone score and bone mineral density in patients with long-term controlled acromegaly. Clin Endocrinol 95(1):58–64. https://doi.org/10.1111/cen.14439
doi: 10.1111/cen.14439
Chappard D (2010) La microarchitecture du tissu osseux. Bulletin De L’académie Nationale De Médecine 194(8):1469–1481. https://doi.org/10.1016/S0001-4079(19)32177-6
doi: 10.1016/S0001-4079(19)32177-6
pubmed: 22046710
Costain DJ, Crawford RW (2009) Fresh-frozen versus irradiated allograft bone in orthopaedic reconstructive surgery. Injury 40(12):1260–1264. https://doi.org/10.1016/j.injury.2009.01.116
doi: 10.1016/j.injury.2009.01.116
pubmed: 19486972
Ding M, Overgaard S (2021) 3-D Microarchitectural properties and rod- and plate-like trabecular morphometric properties of femur head cancellous bones in patients with rheumatoid arthritis, osteoarthritis, and osteoporosis. J Orthop Transl 28:159–168. https://doi.org/10.1016/j.jot.2021.02.002
doi: 10.1016/j.jot.2021.02.002
Domander R, Felder AA, Doube M (2021) BoneJ2: refactoring established research software. Wellcome Open Res 6:37. https://doi.org/10.12688/wellcomeopenres.16619.2
doi: 10.12688/wellcomeopenres.16619.2
pubmed: 33954267
pmcid: 8063517
Follet H, Bruyère-Garnier K, Peyrin F, Roux JP, Arlot ME, Burt-Pichat B, Rumelhart C, Meunier PJ (2005) Relationship between compressive properties of human os calcis cancellous bone and microarchitecture assessed from 2D and 3D synchrotron microtomography. Bone 36(2):340–351. https://doi.org/10.1016/j.bone.2004.10.011
doi: 10.1016/j.bone.2004.10.011
pubmed: 15780961
Fölsch C, Mittelmeier W, Bilderbeek U, Timmesfeld N, von Garrel T, Matter HP (2012) Effect of storage temperature on allograft bone. Transfus Med Hemother 39(1):36–40. https://doi.org/10.1159/000335647
doi: 10.1159/000335647
pubmed: 22896765
Fölsch C, Mittelmeier W, von Garrel T, Bilderbeek U, Timmesfeld N, Pruss A, Matter H-P (2015) Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone. Cell Tissue Bank 16(1):73–81. https://doi.org/10.1007/s10561-014-9442-0
doi: 10.1007/s10561-014-9442-0
pubmed: 24692177
Engelkes K (2021) Accuracy of bone segmentation and surface generation strategies analyzed by using synthetic CT volumes. J Anat 238(6):1456–1471. https://doi.org/10.1111/joa.13383
doi: 10.1111/joa.13383
pubmed: 33325545
Greenwood C, Clement J, Dicken A, Evans P, Iain Lyburn M, Martin NS, Zioupos P, Rogers K (2018) Age-related changes in femoral head trabecular microarchitecture. Aging Dis 9(6):12
doi: 10.14336/AD.2018.0124
Johannesdottir F, Aspelund T, Reeve J, Poole KE, Sigurdsson S, Harris TB, Gudnason VG, Sigurdsson G (2013) Similarities and differences between sexes in regional loss of cortical and trabecular bone in the mid-femoral neck: the ages-Reykjavik longitudinal study. J Bone Miner Res: off J Am Soc Bone Miner Res 28(10):2165–2176. https://doi.org/10.1002/jbmr.1960
doi: 10.1002/jbmr.1960
Lakhwani Op, Jindal M, Kaur O, Chandoke Rk, Kapoor Sk (2017) Effect of bone bank processing on bone mineral density, histomorphometry & biomechanical strength of retrieved femoral head. Indian J Med Res 146(8):45. https://doi.org/10.4103/ijmr.IJMR_739_15
doi: 10.4103/ijmr.IJMR_739_15
Lee W, Jasiuk I (2014) Effects of freeze-thaw and micro-computed tomography irradiation on structure-property relations of porcine trabecular bone. J Biomech 47(6):1495–1498. https://doi.org/10.1016/j.jbiomech.2014.02.022
doi: 10.1016/j.jbiomech.2014.02.022
pubmed: 24612985
Matter HP, Garrel TV, Bilderbeek U, Mittelmeier W (2001) Biomechanical examinations of cancellous bone concerning the influence of duration and temperature of cryopreservation. J Biomed Mater Res 55(1):40–44
doi: 10.1002/1097-4636(200104)55:1<40::AID-JBM60>3.0.CO;2-6
pubmed: 11426396
Mazurkiewicz AJ (2018) The effect of trabecular bone storage method on its elastic properties. Acta Bioeng Biomech 20(1):21–27. https://doi.org/10.5277/ABB-00967-2017-03
doi: 10.5277/ABB-00967-2017-03
pubmed: 29658529
Metzner F, Neupetsch C, Fischer J-P, Drossel W-G, Heyde C-E, Schleifenbaum S (2021) Influence of osteoporosis on the compressive properties of femoral cancellous bone and its dependence on various density parameters. Sci Rep 11(1):13284. https://doi.org/10.1038/s41598-021-92685-z
doi: 10.1038/s41598-021-92685-z
pubmed: 34168240
pmcid: 8225914
Mirzaali F, Libonati C, Böhm L, Rinaudo BM, Cesana FM, Ulivieri LV (2020) Fatigue-caused damage in trabecular bone from clinical, morphological and mechanical perspectives. Int J Fatigue 133:105451. https://doi.org/10.1016/j.ijfatigue.2019.105451
doi: 10.1016/j.ijfatigue.2019.105451
Montoya MJ, José Ma, Giner M, Miranda C, Vázquez MA, Caeiro JR, Guede D, Pérez-Cano R (2014) Microstructural trabecular bone from patients with osteoporotic hip fracture or osteoarthritis: its relationship with bone mineral density and bone remodelling markers. Maturitas 79(3):299–305. https://doi.org/10.1016/j.maturitas.2014.07.006
doi: 10.1016/j.maturitas.2014.07.006
pubmed: 25124531
Otsu N (1979) A threshold selection method from gray-level histograms. IEEE Trans Syst Man Cybern 9(1):62–66. https://doi.org/10.1109/TSMC.1979.4310076
doi: 10.1109/TSMC.1979.4310076
Naot D, Watson M, Choi AJ, Musson DS, Callon KE, Zhu M, Gao R et al (2020) The effect of age on the microarchitecture and profile of gene expression in femoral head and neck bone from patients with osteoarthritis. Bone Rep 13:100287. https://doi.org/10.1016/j.bonr.2020.100287
doi: 10.1016/j.bonr.2020.100287
pubmed: 32551338
pmcid: 7292911
Nazarian A, von Stechow D, Zurakowski D, Müller R, Snyder BD (2008) Bone volume fraction explains the variation in strength and stiffness of cancellous bone affected by metastatic cancer and osteoporosis. Calcif Tissue Int 83(6):368–379. https://doi.org/10.1007/s00223-008-9174-x
doi: 10.1007/s00223-008-9174-x
pubmed: 18946628
Perchalski B, Placke A, Sukhdeo SM, Shaw CN, Gosman JH, Raichlen DA, Ryan TM (2018) Asymmetry in the cortical and trabecular bone of the human humerus during development. Anat Rec 301(6):1012–1025. https://doi.org/10.1002/ar.23705
doi: 10.1002/ar.23705
Porrelli D, Abrami M, Pelizzo P, Formentin C, Ratti C, Turco G, Grassi M, Canton G, Grassi G, Murena L (2022) Trabecular bone porosity and pore size distribution in osteoporotic patients—a low field nuclear magnetic resonance and microcomputed tomography investigation. J Mech Behav Biomed Mater 125:104933. https://doi.org/10.1016/j.jmbbm.2021.104933
doi: 10.1016/j.jmbbm.2021.104933
pubmed: 34837800
Renault J-B, Carmona M, Tzioupis C, Ollivier M, Argenson J-N, Parratte S, Chabrand P (2020) Tibial subchondral trabecular bone micromechanical and microarchitectural properties are affected by alignment and osteoarthritis stage. Sci Rep 10(1):3975. https://doi.org/10.1038/s41598-020-60464-x
doi: 10.1038/s41598-020-60464-x
pubmed: 32132556
pmcid: 7055326
Rincón-Kohli L, Zysset PK (2009) Multi-Axial mechanical properties of human trabecular bone. Biomech Model Mechanobiol 8(3):195–208. https://doi.org/10.1007/s10237-008-0128-z
doi: 10.1007/s10237-008-0128-z
pubmed: 18695984
Ryan MK, Oliviero S, Costa MC, Wilkinson JM, Dall’Ara E (2020) Heterogeneous strain distribution in the subchondral bone of human osteoarthritic femoral heads measured with digital volume correlation. Materials 13(20):4619. https://doi.org/10.3390/ma13204619
doi: 10.3390/ma13204619
pubmed: 33081288
pmcid: 7603047
Shaw JM, Hunter SA, Gayton CJ, Boivin GP, Prayson MJ (2012) Repeated freeze-thaw cycles do not alter the biomechanical properties of fibular allograft bone. Clin Orthop Related Res 470(3):937
doi: 10.1007/s11999-011-2033-5
Singh R, Singh D, Singh A (2016) Radiation sterilization of tissue allografts: a review. World J Radiol 8(4):355–369. https://doi.org/10.4329/wjr.v8.i4.355
doi: 10.4329/wjr.v8.i4.355
pubmed: 27158422
pmcid: 4840193
Spin-Neto R, Stavropoulos A, Coletti FL, Faeda RS, Pereira LAVD, Jr Marcantonio E (2014) Graft incorporation and implant osseointegration following the use of autologous and fresh-frozen allogeneic block bone grafts for lateral ridge augmentation. Clin Oral Implant Res 25(2):226–233. https://doi.org/10.1111/clr.12107
doi: 10.1111/clr.12107
Suto K, Urabe K, Naruse K, Uchida K, Matsuura T, Mikuni-Takagaki Y, Suto M, Nemoto N, Kamiya K, Itoman M (2012) Repeated freeze-thaw cycles reduce the survival rate of osteocytes in bone-tendon constructs without affecting the mechanical properties of tendons. Cell Tissue Bank 13(1):71–80. https://doi.org/10.1007/s10561-010-9234-0
doi: 10.1007/s10561-010-9234-0
pubmed: 21116722
Uchiyama T, Tanizawa T, Muramatsu H, Endo N, Takahashi HE, Hara T (1999) Three-dimensional microstructural analysis of human trabecular bone in relation to its mechanical properties. Bone 25(4):487–491. https://doi.org/10.1016/s8756-3282(99)00188-x
doi: 10.1016/s8756-3282(99)00188-x
pubmed: 10511117
Vastel L, Meunier A, Siney H, Sedel L, Courpied J-P (2004) Effect of different sterilization processing methods on the mechanical properties of human cancellous bone allografts. Biomaterials 25(11):2105–2110. https://doi.org/10.1016/j.biomaterials.2003.08.067
doi: 10.1016/j.biomaterials.2003.08.067
pubmed: 14741625
Villatte G, Erivan R, Descamps S, Arque P, Boisgard S, Wittrant Y (2022) In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts. PLoS ONE 7(10):e0275480. https://doi.org/10.1371/journal.pone.0275480
doi: 10.1371/journal.pone.0275480