Experimental vaccination by single dose sporozoite injection of blood-stage attenuated malaria parasites.
Plasmodium
Genetic Attenuation
Malaria
Vaccine
Virulence
Journal
EMBO molecular medicine
ISSN: 1757-4684
Titre abrégé: EMBO Mol Med
Pays: Germany
ID NLM: 101487380
Informations de publication
Date de publication:
05 Aug 2024
05 Aug 2024
Historique:
received:
27
04
2023
accepted:
02
07
2024
revised:
28
06
2024
medline:
6
8
2024
pubmed:
6
8
2024
entrez:
5
8
2024
Statut:
aheadofprint
Résumé
Malaria vaccination approaches using live Plasmodium parasites are currently explored, with either attenuated mosquito-derived sporozoites or attenuated blood-stage parasites. Both approaches would profit from the availability of attenuated and avirulent parasites with a reduced blood-stage multiplication rate. Here we screened gene-deletion mutants of the rodent parasite P. berghei and the human parasite P. falciparum for slow growth. Furthermore, we tested the P. berghei mutants for avirulence and resolving blood-stage infections, while preserving sporozoite formation and liver infection. Targeting 51 genes yielded 18 P. berghei gene-deletion mutants with several mutants causing mild infections. Infections with the two most attenuated mutants either by blood stages or by sporozoites were cleared by the immune response. Immunization of mice led to protection from disease after challenge with wild-type sporozoites. Two of six generated P. falciparum gene-deletion mutants showed a slow growth rate. Slow-growing, avirulent P. falciparum mutants will constitute valuable tools to inform on the induction of immune responses and will aid in developing new as well as safeguarding existing attenuated parasite vaccines.
Identifiants
pubmed: 39103697
doi: 10.1038/s44321-024-00101-6
pii: 10.1038/s44321-024-00101-6
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsches Zentrum für Infektionsforschung (DZIF)
ID : TTU 03.813
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : FR2140/10-1
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : SFB 1129
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : FR2140/6-1
Organisme : Volkswagen Foundation (VolkswagenStiftung)
ID : Az 92393
Informations de copyright
© 2024. The Author(s).
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