Stress response in Escherichia coli following sublethal phenalene-1-one mediated antimicrobial photodynamic therapy: an RNA-Seq study.

Escherichia coli Antibacterial RNA-Seq Stress response Transcriptomics aPDT

Journal

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
ISSN: 1474-9092
Titre abrégé: Photochem Photobiol Sci
Pays: England
ID NLM: 101124451

Informations de publication

Date de publication:
05 Aug 2024
Historique:
received: 22 01 2024
accepted: 20 07 2024
medline: 6 8 2024
pubmed: 6 8 2024
entrez: 5 8 2024
Statut: aheadofprint

Résumé

Since the molecular mechanisms behind adaptation and the bacterial stress response toward antimicrobial photodynamic therapy (aPDT) are not entirely clear yet, the aim of the present study was to investigate the transcriptomic stress response in Escherichia coli after sublethal treatment with aPDT using RNA sequencing (RNA-Seq). Planktonic cultures of stationary phase E. coli were treated with aPDT using a sublethal dose of the photosensitizer SAPYR. After treatment, RNA was extracted, and RNA-Seq was performed on the Illumina NextSeq 500. Differentially expressed genes were analyzed and validated by qRT-PCR. Furthermore, expression of specific stress response proteins was investigated using Western blot analysis.The analysis of the differential gene expression following pathway enrichment analysis revealed a considerable number of genes and pathways significantly up- or down-regulated in E. coli after sublethal treatment with aPDT. Expression of 1018 genes was up-regulated and of 648 genes was down-regulated after sublethal treatment with aPDT as compared to irradiated controls. Analysis of differentially expressed genes and significantly de-regulated pathways showed regulation of genes involved in oxidative stress response and bacterial membrane damage. In conclusion, the results show a transcriptomic stress response in E. coli upon exposure to aPDT using SAPYR and give an insight into potential molecular mechanisms that may result in development of adaptation.

Identifiants

pubmed: 39103724
doi: 10.1007/s43630-024-00617-3
pii: 10.1007/s43630-024-00617-3
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : CI 263/1-3

Informations de copyright

© 2024. The Author(s).

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Auteurs

Denise Muehler (D)

Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.

Silvia Morini (S)

Quantitative Biology Center (QBiC), University of Tübingen, Tübingen, Germany.

Janina Geißert (J)

NGS-Competence Center Tübingen, Institute for Medical Microbiology and Hygiene, University Hospital Tübingen, Tübingen, Germany.

Christina Engesser (C)

NGS-Competence Center Tübingen, Institute for Medical Microbiology and Hygiene, University Hospital Tübingen, Tübingen, Germany.

Karl-Anton Hiller (KA)

Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.

Matthias Widbiller (M)

Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.

Tim Maisch (T)

Department of Dermatology, University Hospital Regensburg, Regensburg, Germany.

Wolfgang Buchalla (W)

Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.

Fabian Cieplik (F)

Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany. fabian.cieplik@uniklinik-freiburg.de.
Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center - University of Freiburg, Medical Faculty, University of Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany. fabian.cieplik@uniklinik-freiburg.de.

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