The ORP9-ORP11 dimer promotes sphingomyelin synthesis.
Lipidomics
ORP11
ORP9
Sphingomyelin
biochemistry
cell biology
chemical biology
human
lipid transfer proteins
membrane contact sites
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
06 Aug 2024
06 Aug 2024
Historique:
medline:
6
8
2024
pubmed:
6
8
2024
entrez:
6
8
2024
Statut:
epublish
Résumé
Numerous lipids are heterogeneously distributed among organelles. Most lipid trafficking between organelles is achieved by a group of lipid transfer proteins (LTPs) that carry lipids using their hydrophobic cavities. The human genome encodes many intracellular LTPs responsible for lipid trafficking and the function of many LTPs in defining cellular lipid levels and distributions is unclear. Here, we created a gene knockout library targeting 90 intracellular LTPs and performed whole-cell lipidomics analysis. This analysis confirmed known lipid disturbances and identified new ones caused by the loss of LTPs. Among these, we found major sphingolipid imbalances in ORP9 and ORP11 knockout cells, two proteins of previously unknown function in sphingolipid metabolism. ORP9 and ORP11 form a heterodimer to localize at the ER-
Identifiants
pubmed: 39106189
doi: 10.7554/eLife.91345
pii: 91345
doi:
pii:
Substances chimiques
Sphingomyelins
0
oxysterol binding protein
0
Carrier Proteins
0
Receptors, Steroid
0
Phosphatidylinositol Phosphates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : Spinoza 00897590
Informations de copyright
© 2023, Cabukusta et al.
Déclaration de conflit d'intérêts
BC, SB, JA, NB, AM, RK, MG, JN No competing interests declared
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