The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare).
ABA
Alternative splicing
Barley
Cap-binding complex
Embryo
Germination
Transcriptome
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 Aug 2024
07 Aug 2024
Historique:
received:
15
03
2024
accepted:
05
08
2024
medline:
7
8
2024
pubmed:
7
8
2024
entrez:
6
8
2024
Statut:
epublish
Résumé
To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.
Identifiants
pubmed: 39107424
doi: 10.1038/s41598-024-69373-9
pii: 10.1038/s41598-024-69373-9
doi:
Substances chimiques
Abscisic Acid
72S9A8J5GW
Plant Proteins
0
RNA Cap-Binding Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18278Subventions
Organisme : Narodowe Centrum Nauki
ID : 2020/38/E/NZ9/00346
Informations de copyright
© 2024. The Author(s).
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