Comprehensive analysis of L-PRF exudate components and their impact on whole blood platelets.

Humans Male Cytokines / metabolism Flow Cytometry Blood Platelets Female Adult Platelet-Rich Fibrin Exudates and Transudates Healthy Volunteers Platelet Activation Leukocytes Biomarkers / blood

Flow cytometry Inflammatory cytokines L-PRF exudate Leukocyte subpopulations Platelet activation

Journal

Clinical oral investigations

ISSN: 1436-3771

Titre abrégé: Clin Oral Investig

Pays: Germany

ID NLM: 9707115

Informations de publication

Date de publication:
07 Aug 2024

Historique:

received: 28 11 2023

accepted: 31 07 2024

medline: 7 8 2024

pubmed: 7 8 2024

entrez: 7 8 2024

Statut: epublish

Résumé

This study assessed the cellular composition and effects of leukocyte-platelet-rich fibrin (L-PRF) exudate on whole blood platelets from healthy volunteers. Key objectives included evaluating leukocyte subpopulations, platelet activation markers, platelet-leukocyte interactions and quantifying inflammatory cytokines within the L-PRF exudate. L-PRF was obtained from 20 healthy donors. Flow cytometry methodologies were used to assess intracellular calcium kinetics and activated GPIIbIIIa, and P-selectin expression. Leukocyte subpopulations and platelet-leukocyte interactions were characterized using monoclonal antibodies. Inflammatory cytokines (IL-8, IL-1β, IL-6, IL-10, TNF, IL-12p70) within L-PRF exudate were quantified using a cytometric bead array. The expression of activated GPIIbIIIa, and P-selectin exhibited a significant increase (p < 0.001) when L-PRF exudate was added to platelets of whole blood. Regarding intracellular Ca Despite the study limitations, the research yielded important insights: 1- L-PRF exudate can stimulate platelet activation, essential in healing, tissue inflammation and remodeling. 2-The presence of leukocyte subpopulations within L-PRF exudate reflexes its complexity and potential to enhance immune responses. 3-The analysis of inflammatory cytokines within L-PRF exudate revealed its immunomodulatory potential. These findings are valuable evidences for understanding the potential role of L-PRF exudate in regenerative dentistry and medicine, offering innovative therapeutic strategies. This research highlights crucial aspects that could significantly influence the clinical use of L-PRF exudate in the oral cavity. The findings support the application of L-PRF exudate in both surgical and regenerative dentistry, facilitating the development of innovative therapeutic strategies to enhance patient outcomes.

Identifiants

DOI: 10.1007/s00784-024-05868-z PMID: 39110266

pubmed: 39110266

doi: 10.1007/s00784-024-05868-z

pii: 10.1007/s00784-024-05868-z

doi:

Substances chimiques

Cytokines 0

Biomarkers 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

470

Subventions

Organisme : UNIPRO - Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU and 1H-TOXRUN - One Health Toxicology Research Unit, University Institute of Health Sciences (IUCS), CESPU

ID : PAAALPRF_PI2RL_IINFACTS_2021

Informations de copyright

Références

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Comprehensive analysis of L-PRF exudate components and their impact on whole blood platelets.

Journal

Informations de publication

Résumé

Identifiants

Substances chimiques

Types de publication

Langues

Sous-ensembles de citation

Pagination

Subventions

Informations de copyright

Références

Auteurs

António Melo-Ferraz (A)

Cristina Coelho (C)

Paulo Miller (P)

Maria Begoña Criado (MB)

Maria Céu Monteiro (MC)

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