Culture positivity and distribution of the conjunctival sac bacteria in the perioperative period of corneal refractive surgery.
Humans
Conjunctiva
/ microbiology
Male
Female
Refractive Surgical Procedures
/ adverse effects
Adult
Staphylococcus epidermidis
/ isolation & purification
Middle Aged
Perioperative Period
Anti-Bacterial Agents
/ therapeutic use
Cornea
/ microbiology
Young Adult
Ophthalmic Solutions
Antibiotic Prophylaxis
/ methods
Bacteria
/ isolation & purification
Bacterial culture
Conjunctival sac
Perioperative
Refractive surgery
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 Aug 2024
07 Aug 2024
Historique:
received:
22
04
2024
accepted:
31
07
2024
medline:
8
8
2024
pubmed:
8
8
2024
entrez:
7
8
2024
Statut:
epublish
Résumé
The objective of this study was to investigate the culture positivity and distribution of the conjunctival sac bacteria in the perioperative period of corneal refractive surgery. The selected time points of the perioperative period included before the use of antibiotic eye drops, before eye wash (after the use of antibiotic eye drops), after eye wash, and immediately after surgery. Conjunctival specimens obtained at the four time points were cultured to detect the positivity and distribution of bacteria. Before prophylactic antibiotic eye drops were administered, 49 eyes (50%) had positive bacterial culture results, with 45 isolates (91.8%) identified as Staphylococcus epidermidis. The culture positivity rates of the conjunctival sac specimens before eye wash, after eye wash, and immediately after surgery were 19.4%, 3.1%, and 4.1%, respectively. The difference was significant before and after the use of antibiotics and before and after eye wash (both P < 0.001). Staphylococcus epidermidis was the major pathogen in the conjunctival sac before corneal refractive surgery, and the culture positivity rate of the conjunctival bacteria was higher in males. Sixteen of 37 eyes (43.2%) with contact lenses had positive culture results, compared to 33 of 61 eyes (54.1%) without contact lenses (P > 0.05). The judicious preoperative use of antibiotic eye drops combined with the surgical sterile eye wash procedure maximised the removal of conjunctival sac bacteria. Skilled surgical manipulations generally did not increase the risk of infection.
Identifiants
pubmed: 39112522
doi: 10.1038/s41598-024-69060-9
pii: 10.1038/s41598-024-69060-9
doi:
Substances chimiques
Anti-Bacterial Agents
0
Ophthalmic Solutions
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18364Subventions
Organisme : Shandong Province Medical and Health Technology Development Plan Project
ID : 202207020689
Informations de copyright
© 2024. The Author(s).
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