Antidiarrheal, anti-inflammatory and antioxidant effects of Anethum graveolens L. fruit extract on castor oil-induced diarrhea in rats.
Anethum gravelons L.
Castor oil
diarrhea
inflammation
nutraceutical
oxidative stress
Journal
Neurogastroenterology and motility
ISSN: 1365-2982
Titre abrégé: Neurogastroenterol Motil
Pays: England
ID NLM: 9432572
Informations de publication
Date de publication:
08 Aug 2024
08 Aug 2024
Historique:
revised:
04
07
2024
received:
20
12
2023
accepted:
29
07
2024
medline:
8
8
2024
pubmed:
8
8
2024
entrez:
8
8
2024
Statut:
aheadofprint
Résumé
Between food and medicine, nutraceuticals are widely used in human health for the prevention and treatment of various diseases. This study aims to determine the cytoprotective effects of Anethum gravelons fruit extract (AGFAE) on castor oil-induced diarrhea in rats due to its phytochemical and antioxidant properties. Male rats were divided into six groups of six animals each: Control (C), Castor oil (CO), CO + different doses of AGFAE (50, 100, and 200 mg/kg, b.w., p.o.), and the CO + loperamide group (LOP, 10 mg/kg, b.w., p.o.). In vitro, the chemical composition of aqueous Dill fruit extract showed strong antioxidant activity, with a high content of total polyphenols, flavonoids, and tannins. In our in vivo studies, pre-treatment with AGFAE reduced malondialdehyde and hydrogen peroxide levels and maintained normal activity of enzymatic and non-enzymatic antioxidants in the gastric and intestinal mucosa. In addition, we found that AGFAE prophylaxis improved the stability of many plasma biochemical parameters altered by castor oil intoxication, such as C-reactive protein concentrations and alkaline phosphatase activities. We suggest that AGFAE phenolic compounds had significant protection against diarrhea involving several mechanisms such as reducing hypersecretion, peristaltic, inflammation, and preserving the endogenous antioxidant levels.
Sections du résumé
BACKGROUND
BACKGROUND
Between food and medicine, nutraceuticals are widely used in human health for the prevention and treatment of various diseases. This study aims to determine the cytoprotective effects of Anethum gravelons fruit extract (AGFAE) on castor oil-induced diarrhea in rats due to its phytochemical and antioxidant properties.
METHODS
METHODS
Male rats were divided into six groups of six animals each: Control (C), Castor oil (CO), CO + different doses of AGFAE (50, 100, and 200 mg/kg, b.w., p.o.), and the CO + loperamide group (LOP, 10 mg/kg, b.w., p.o.).
KEY RESULTS
RESULTS
In vitro, the chemical composition of aqueous Dill fruit extract showed strong antioxidant activity, with a high content of total polyphenols, flavonoids, and tannins. In our in vivo studies, pre-treatment with AGFAE reduced malondialdehyde and hydrogen peroxide levels and maintained normal activity of enzymatic and non-enzymatic antioxidants in the gastric and intestinal mucosa. In addition, we found that AGFAE prophylaxis improved the stability of many plasma biochemical parameters altered by castor oil intoxication, such as C-reactive protein concentrations and alkaline phosphatase activities.
CONCLUSIONS & INFERENCES
CONCLUSIONS
We suggest that AGFAE phenolic compounds had significant protection against diarrhea involving several mechanisms such as reducing hypersecretion, peristaltic, inflammation, and preserving the endogenous antioxidant levels.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14892Informations de copyright
© 2024 The Author(s). Neurogastroenterology & Motility published by John Wiley & Sons Ltd.
Références
Maikere‐Faniyo RVPL, Mutwewingabo A, Habiyaremye FX. Study of Rwandese medicinal plants used in the treatment of diarrhoea I. J Ethnopharmacol. 1989;26(2):101‐109.
Bahekar SE, Kale RS. Antidiarrheal activity of ethanolic extract of Manihot esculenta Crantz leaves in Wistar rats. J Ayurveda Integr Med. 2015;6(1):35‐40.
Food and Agriculture Organization TiMP. Raw Materials, Tropical and Horticultural Products Service Commodities and Trade Division Economic and Social Department. 2008.
WHO. Essential Medicine and Health Products Information Portal, A World Health Organization resource. 1993.
Carbonell‐Capella JM, Barba FJ, Esteve MJ, Frígola A. High pressure processing of fruit juice mixture sweetened with Stevia rebaudiana Bertoni: optimal retention of physical and nutritional quality. IFSET. 2013;18:48‐56.
Daliu P, Santini A, Novellino E. From pharmaceuticals to nutraceuticals: bridging disease prevention and management. Expert Rev Clin Pharmacol. 2019;12(1):1‐7.
Kiani M‐A, Khodadad A, Mohammadi S, et al. Effect of peppermint on pediatrics' pain under endoscopic examination of the large bowel. J HerbMed Pharmacol. 2013;2(2):41‐44.
Hosseini‐Asl K, Rafieian‐Kopaei M. Can patients with active duodenal ulcer fast Ramadan? Am J Gastroenterol. 2002;97(9):2471‐2472.
Nasri H, Baradaran A, Shirzad H, Rafieian‐Kopaei M. New concepts in nutraceuticals as alternative for pharmaceuticals. Int J Prev Med. 2014;5(12):1487‐1499.
AlAli M, Alqubaisy M, Aljaafari MN, et al. Nutraceuticals: transformation of conventional foods into health promoters/disease preventers and safety considerations. Molecules. 2021;26(9):2540.
Zeyneb TB, Fawzia TB, Mohamed B, Joon‐Kwan M. Antifungal activity and evaluation of phenolics contents of dill Anethum graveolens L. extracts original from Algeria. Indian J Nat Prod Resour. 2020;10(3):188‐194.
Brinsi C, Selmi H, Jedidi S, Sebai H. Enquête ethnopharmacologique sur l'usage traditionnel de l'aneth (Anethum graveolens L.) dans le nord‐ouest de la tunisie. Revue Marocaine Des Sciences Agronomiques et Vétérinaires. 2022;10(2):282‐286.
Ishikawa T, Kudo M, Kitajima J. Water‐soluble constituents of dill. Chem Pharm Bull. 2002;50(4):501‐507.
Hajhashemi V, Abbasi N. Hypolipidemic activity of Anethum graveolens in rats. Phytother Res. 2008;22(3):372‐375.
Ravindra S. Agro‐Techniques of Medicinal Plants. Daya Publishing House; 2004.
Pilly EÉO, le Berre R, Tattevin P. Collège des universitaires de maladies infectieuses et tropicales (France). ECNPilly: maladies infectieuses et tropicales : préparation ECN, tous les items d'infectiologie. Alinéa Plus Edition; 2017.
Carré D. Conduite à tenir devant une diarrhée aiguë. Étiologies EMC‐Chirurgie. 2004;1(5):493‐532.
Abergel A, Baumert T, Seksik P, et al. Hépato‐gastro‐entérologie‐Chirurgie digestive: Réussir les ECNi. Elsevier Health Sciences; 2018.
Feldman M, Scharschmidt B, Sleisenger MH. Gastrointestinal and Liver Disease. Endoskopie Heute. 1998;11:221.
Geboes K, De Hertogh G, Ectors N. Drug‐induced pathology in the large intestine. Curr. Diagn. Pathol. 2006;12(4):239‐247.
Huijghebaert S, Awouters F, Tytgat G. Racecadotril versus loperamide: antidiarrheal research revisited. Dig Dis Sci. 2003;48:239‐250.
van Pv S, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991;74(10):3583‐3597.
Vl S. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin‐Ciocalteu reagent. Methods Enzymol. 1999;299:152‐178.
Yi Z, Yu Y, Liang Y, Zeng B. In vitro antioxidant and antimicrobial activities of the extract of Pericarpium Citri Reticulatae of a new citrus cultivar and its main flavonoids. LWT. 2008;41(4):597‐603.
Kujala TS, Loponen JM, Klika KD, Pihlaja K. Phenolics and betacyanins in red beetroot (Beta v ulgaris) root: distribution and effect of cold storage on the content of total phenolics and three individual compounds. J Agric Food Chem. 2000;48(11):5338‐5342.
Ammar RB, Bhouri W, Sghaier MB, et al. Antioxidant and free radical‐scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L.(Rhamnaceae): a structure‐activity relationship study. Food Chem. 2009;116(1):258‐264.
Organization WH. Research Guidelines for Evaluating the Safety and Efficacy of Herbal Medicines. Regional Office for Western Pacific Manila; 1993:1‐2.
Awouters F, Niemegeers C, Lenaerts F, Janssen P. Delay of castor oil diarrhoea in rats: a new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharm Pharmacol. 1978;30(1):41‐45.
Mukherjee PK, Saha K, Murugesan T, Mandal S, Pal M, Saha B. Screening of anti‐diarrhoeal profile of some plant extracts of a specific region of West Bengal, India. J Ethnopharmacol. 1998;60(1):85‐89.
Carlo GD, Mascolo N, Izzo AA, Capasso F, Autore G. Effects of quercetin on the gastrointestinal tract in rats and mice. Phytother Res. 1994;8(1):42‐45.
Ali B, Bashir A. The effect of some α2‐adrenoceptor agonists and antagonists on gastrointestinal transit in mice: influence of morphine, castor oil and glucose. Clin Exp Pharmacol Physiol. 1993;20(1):1‐6.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein‐dye binding. Anal Biochem. 1976;72(1–2):248‐254.
Draper HH, Hadley M. [43] malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology. Vol 186. Elsevier; 1990:421‐431.
Dingeon B, Ferry J, Roullet A. Automatic assay of blood sugar by Trinder's method. Ann Biol Clin (Paris). 1975;33(1):3‐13.
Kakkar PDB, Viswanathan PN. Modified spectrophotometric assay of SOD. Indian J Biochem Biophys. 1984;2:130‐132.
Aebi H. Catalase in vitro. Methods in Enzymology. Vol 105. Elsevier; 1984:121‐126.
Flohé L, Günzler WA. Assays of glutathione peroxidase. Methods in Enzymology. Vol 105. Elsevier; 1984:114‐120.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70‐77.
Heywood VH. Biology and Chemistry of the Umbelliferae. Academic Press; 1971.
Jinesh V, Jaishree V, Badami S, Shyam W. Comparative Evaluation of Antioxidant Properties of Edible and Non‐edible Leaves of Anethum Graveolens Linn. Council of Scientific & Industrial Research (CSIR); 2010.
Satyanarayana S, Sushruta K, Sarma G, Srinivas N, Raju GS. Antioxidant activity of the aqueous extracts of spicy food additives—evaluation and comparison with ascorbic acid in in vitro systems. J Herb Pharmacother. 2004;4(2):1‐10.
Hosseinzadeh H, Karimi GR, Ameri M. Effects of Anethum graveolens L. seed extracts on experimental gastric irritation models in mice. BMC Pharmacol. 2002;2:1‐5.
Sahib AS, Mohammad I, Al‐Gareeb A. Effects of anethum graveolens leave powder on lipid profile in hyperlipidemic patients. Spatula DD. 2012;2(3):153‐158.
Racusen L, Binder H. Ricinoleic acid stimulation of active anion secretion in colonic mucosa of the rat. J Clin Invest. 1979;63(4):743‐749.
Capasso F, Mascolo N, Izzo AA, Gaginella TS. Dissociation of castor oil‐induced diarrhoea and intestinal mucosal injury in rat: effect of NG‐nitro‐L‐arginine methyl ester. Br J Pharmacol. 1994;113(4):1127‐1130.
Imam MZ, Sultana S, Akter S. Antinociceptive, antidiarrheal, and neuropharmacological activities of Barringtonia acutangula. Pharm Biol. 2012;50(9):1078‐1084.
Pintoy A, Autore G, Mascolo N, et al. Time course of PAF formation by gastrointestinal tissue in rats after Castor oil challenge. J Pharm Pharmacol. 1992;44(3):224‐226.
Mascolo N, Izzo AA, Autore G, Barbato F, Capasso F. Nitric oxide and castor oil‐induced diarrhea. J Pharmacol Exp Ther. 1994;268(1):291‐295.
Robert A, Nezamis J, Lancaster C, Hanchar A, Klepper M. Enteropooling assay: a test for diarrhea produced by prostaglandins. Prostaglandins. 1976;11(5):809‐828.
Chanzy S, Moretti S, Mayet H, Routon M, de Gennes C, Mselati J. Loss of consciousness in a child due to loperamide. Arch Pediatr. 2004;11(7):826‐827.
Cézard JPCJ, Girardet JP, Gottrand F. Traitement médicamenteux des diarrhées aiguės infectieuses du nourrisson et de l'enfant. Arch Pediatr. 2002;9(6):620‐628.
Sebai H, Jabri M‐A, Souli A, et al. Antidiarrheal and antioxidant activities of chamomile (Matricaria recutita L.) decoction extract in rats. J Ethnopharmacol. 2014;152(2):327‐332.
de la Lastra CA, Martin MJ, la Casa C, Motilva V. Antiulcerogenicity of the flavonoid fraction from Bidens aurea: comparison with ranitidine and omeprazole. J Ethnopharmacol. 1994;42(3):161‐168.
Reyes M, Martín C, de la Lastra A, Trujillo J, Toro MV, Ayuso MJ. Antiulcerogenicity of the flavonoid fraction from Erica andevalensis Cabezudo‐Rivera. Z Naturforsch C J Biosci. 1996;51(7–8):563‐569.
Alvarez A, Pomar F, Sevilla M, Montero M. Gastric antisecretory and antiulcer activities of an ethanolic extract of Bidens pilosa L. var. radiata Schult. Bip. J Ethnopharmacol. 1999;67(3):333‐340.
Mahran G, Kadry H, Thabet C, et al. GC/MS analysis of volatile oil of fruits of Anethum graveolens. Int J Pharmacogn. 1992;30(2):139‐144.
Möhle B, Heller W, Wellmann E. UV‐induced biosynthesis of quercetin 3‐O‐β‐D‐glucuronide in dill cell cultures. Phytochemistry. 1985;24(3):465‐467.
Bezanger‐Beauquesne LPM, Torck M, Trotin F. Plantes médicinales des régions tempérées, (Ed) Maloine. France; 1990:395.
Sanders KM, Koh SD, Ro S, Ward SM. Regulation of gastrointestinal motility—insights from smooth muscle biology. Nat Rev Gastroenterol Hepatol. 2012;9(11):633‐645.
Goldberg M, Robertson D. Yohimbine: a Pharmacological Probe for Study of the Alpha 2‐Adrenoreceptor. Pharmacol. Rev. 1983;35(3):143‐180.
Fleming T. PDR for Herbal Medicines: from Medical Economics Company. New Jersy; 2000:253.
Duraffourd CHL, Lapraz JC. Cahiers de phytothérapie clinique: affections rhumatismales, affections diqestives. Vol 87. 2nd ed. Masson; 1990.
Sammari H, Jedidi S, Selmi H, et al. Protective effects of Crataegus azarolus L. berries aqueous extract against castor oil–induced diarrhea, oxidative stress, and inflammation in rat. Neurogastroenterol Motil. 2021;33(6):e14065.
Baudin B. Stress oxydant et protections antioxydantes. Rev Francoph Lab. 2020;2020(522):22‐30.
Pasquier C. Stress oxydatif et inflammation. Rev Francoph Lab. 1995;1995(276):87‐92.
Jedidi S, Rtibi K, Selmi S, et al. Phytochemical/antioxidant properties and individual/synergistic actions of Salvia officinalis L. aqueous extract and loperamide on gastrointestinal altering motor function. J Med Food. 2019;22(12):1235‐1245.
Rao CV, Vijayakumar M, Sairam K, Kumar V. Antidiarrhoeal activity of the standardised extract of Cinnamomum tamala in experimental rats. J Nat Med. 2008;62:396‐402.
Nguyen M, Nguyen V, Le V, et al. Assessment of preliminary phytochemical screening, polyphenol content, flavonoid content, and antioxidant activity of custard apple leaves (Annona squamosa Linn.). IOP Conference Series: Materials Science and Engineering; IOP Publishing; 2020.
Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010;2(12):1231‐1246.
Cook NL, Viola HM, Sharov VS, Hool LC, Schöneich C, Davies MJ. Myeloperoxidase‐derived oxidants inhibit sarco/endoplasmic reticulum Ca2+‐ATPase activity and perturb Ca2+ homeostasis in human coronary artery endothelial cells. Free Radic Biol Med. 2012;52(5):951‐961.
Belemtougri R, Constantin B, Cognard C, Raymond G, Sawadogo L. Effects of two medicinal plants Psidium guajava L.(Myrtaceae) and Diospyros mespiliformis L.(Ebenaceae) leaf extracts on rat skeletal muscle cells in primary culture. J Zhejiang Univ Sci B. 2006;7:56‐63.
Azadmard‐Damirchi S, Bf A, Alirezalu K, Alirezalu A, Hesari J, Emami S. Physiological and medicinal properties of Castor oil. RPMP. Studium Press; 2012.
Madani H, Mahmoodabady NA, Vahdati A. Effects of hydroalchoholic extract of Anethum graveolens (dill) on plasma glucose and lipid levels in diabetes induced rats. Iranian Journal of Diabetes and Lipid Disorders. 2005;5:109‐116.
Jana S, Shekhawat GS. Anethum graveolens: an Indian traditional medicinal herb and spice. Pharmacogn Rev. 2010;4(8):179‐184.
Yahfoufi N, Alsadi N, Jambi M, Matar C. The immunomodulatory and anti‐inflammatory role of polyphenols. Nutrients. 2018;10(11):1618.
Nagai R, Murray DB, Metz TO, Baynes JW. Chelation: a fundamental mechanism of action of AGE inhibitors, AGE breakers, and other inhibitors of diabetes complications. Diabetes. 2012;61(3):549‐559.
Javad H, Seyed‐Mostafa H‐z, Farhad O, et al. Hepatoprotective effects of hydroalcoholic extract of Allium hirtifolium (Persian shallot) in diabetic rats. J Basic Clin Physiol Pharmacol. 2012;23(2):83‐87.
Isopencu G, Ferdeş M. The effect of Anethum graveolens upon the growth of E. Coli. UPB Scientific Bulletin, Series B. 2012;74(3):85‐92.
Hedges L, Lister C. Nutritional attributes of herbs. Crop & food research report. 2007;1891:1‐89.