Analysing carbapenemases in hospital wastewater: Insights from intracellular and extracellular DNA using qPCR and digital PCR.

Antimicrobial resistance Horizontal gene transfer Hospital wastewater Pollution Transformation

Journal

The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500

Informations de publication

Date de publication:
06 Aug 2024
Historique:
received: 23 05 2024
revised: 16 07 2024
accepted: 05 08 2024
medline: 9 8 2024
pubmed: 9 8 2024
entrez: 8 8 2024
Statut: aheadofprint

Résumé

The widespread dissemination of carbapenem-resistant bacteria in wastewater systems, particularly from clinical sources, poses a significant public health risk. This study assessed the concentrations and distributions of extracellular DNA (exDNA) and intracellular DNA (iDNA) harboring carbapenemase genes in wastewater from six tertiary care hospitals in Germany. We collected a total of 36 samples, comprising six biological replicates from each hospital, and analysed them using quantitative real-time PCR (qPCR) and digital PCR (dPCR). The analysis targeted seven carbapenemase genes: bla

Identifiants

DOI: 10.1016/j.scitotenv.2024.175344 PMID: 39117207
pubmed: 39117207
pii: S0048-9697(24)05494-9
doi: 10.1016/j.scitotenv.2024.175344
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

175344

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Auteurs

Tim Erler (T)

Institute of Hygiene and Public Health, University Hospital Bonn, Bonn, Germany.

Felix Droop (F)

Institute of Hygiene and Public Health, University Hospital Bonn, Bonn, Germany.

Christoph Lübbert (C)

Division of Infectious Diseases and Tropical Medicine, Department of Medicine I, Leipzig University Hospital, Leipzig, Germany.

Johannes K Knobloch (JK)

Institute of Medical Microbiology, Virology, and Hygiene, Department for Infection Prevention and Control, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Laura Carlsen (L)

Institute of Medical Microbiology, Virology, and Hygiene, Department for Infection Prevention and Control, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Cihan Papan (C)

Institute of Hygiene and Public Health, University Hospital Bonn, Bonn, Germany.

Thomas Schwanz (T)

Division of Infection Control and Infectious Diseases, University Hospital RWTH Aachen, Aachen, Germany.

Janine Zweigner (J)

Department of Hospital Hygiene and Infection Control, University Hospital of Cologne, Cologne, Germany.

Jennifer Dengler (J)

Integrative Health and Security Management Center, Staff Section Environmental Protection and Sustainability, Jena University Hospital, Jena, Germany.

Marc Hoffmann (M)

Integrative Health and Security Management Center, Staff Section Environmental Protection and Sustainability, Jena University Hospital, Jena, Germany.

Nico T Mutters (NT)

Institute of Hygiene and Public Health, University Hospital Bonn, Bonn, Germany.

Mykhailo Savin (M)

Institute of Hygiene and Public Health, University Hospital Bonn, Bonn, Germany. Electronic address: michael.savin-hoffmeyer@ukbonn.de.

Classifications MeSH