Structural basis for the distinct core-antenna assembly of cryptophyte photosystem II.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 Aug 2024
09 Aug 2024
Historique:
received:
18
01
2024
accepted:
31
07
2024
medline:
10
8
2024
pubmed:
10
8
2024
entrez:
9
8
2024
Statut:
epublish
Résumé
Photosystem II (PSII) catalyzes the light-driven charge separation and water oxidation reactions of photosynthesis. Eukaryotic PSII core is usually associated with membrane-embedded light-harvesting antennae, which greatly increase the absorbance cross-section of the core. The peripheral antennae in different phototrophs vary considerably in protein composition and arrangement. Photosynthetic cryptophytes possess chlorophyll a/c binding proteins (CACs) that serve as their antennae. How these CACs assemble with the PSII core remains unclear. Here, we report the 2.57-Å resolution structure of cryptophyte PSII-CAC purified from cells at nitrogen-limited stationary growth phase. We show that each monomer of the PSII homodimer contains a core complex, six chlorophyll a/c binding proteins (CACs) and a previously unseen chlorophyll-binding protein (termed CAL-II). Six CACs are arranged as a double-layered arc-shaped non-parallel belt, and two such belts attach to the dimeric core from opposite sides. The CAL-II simultaneously interacts with a number of core subunits and five CACs. The distinct organization of CACs and the presence of CAL-II may play a critical role in stabilizing the dimeric PSII-CAC complex under stress conditions. Our study provides mechanistic insights into the assembly and function of the PSII-CAC complex as well as the possible adaptation of cryptophytes in response to environmental stresses.
Identifiants
pubmed: 39122741
doi: 10.1038/s41467-024-51206-y
pii: 10.1038/s41467-024-51206-y
doi:
Substances chimiques
Photosystem II Protein Complex
0
Chlorophyll Binding Proteins
0
Light-Harvesting Protein Complexes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6812Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31930064
Organisme : Chinese Academy of Sciences (CAS)
ID : XDB37020101
Organisme : Chinese Academy of Sciences (CAS)
ID : YSBR-015
Organisme : Chinese Academy of Sciences (CAS)
ID : Y2022038
Informations de copyright
© 2024. The Author(s).
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