Less is more: CRISPR/Cas9-based mutations in DND1 gene enhance tomato resistance to powdery mildew with low fitness costs.
dnd1 mutants
CRISPR/Cas9
Reduced fitness costs
Reduced symptoms
S-genes
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
10 Aug 2024
10 Aug 2024
Historique:
received:
28
03
2024
accepted:
16
07
2024
medline:
10
8
2024
pubmed:
10
8
2024
entrez:
9
8
2024
Statut:
epublish
Résumé
Powdery mildew (PM), triggered by Oidium neolycopersici, represents a significant threat and a major concern for the productivity of tomato plants (Solanum lycopersicum L.). The presence of susceptibility (S) genes in plants facilitates pathogen proliferation and their dysfunction can lead to a recessively inherited broad-spectrum and durable type of resistance. Past studies have demonstrated that disrupting the function of DND1 (Defense No Death 1) increases plant resilience against various pathogens, such as powdery mildew (PM), but this comes at the cost of negatively affecting the overall health and vigor of the plant. To investigate the possibility of minimizing the adverse effects of the dnd1 mutation while boosting disease resistance, a CRISPR-Cas9 construct with four single guide RNAs targeting three exons of SlDND1 (Solyc02g088560.4.1) was designed and introduced into the tomato variety Moneymaker (MM) through Agrobacterium tumefaciens-mediated transformation. Three T
Identifiants
pubmed: 39123110
doi: 10.1186/s12870-024-05428-3
pii: 10.1186/s12870-024-05428-3
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
763Subventions
Organisme : Cassa di Risparmio di Cuneo (CRC) Foundation
ID : PROSPEcT research project, www.crispr-plants.unito.it
Organisme : Cassa di Risparmio di Cuneo (CRC) Foundation
ID : PROSPEcT research project, www.crispr-plants.unito.it
Organisme : Cassa di Risparmio di Cuneo (CRC) Foundation
ID : PROSPEcT research project, www.crispr-plants.unito.it
Organisme : Cassa di Risparmio di Cuneo (CRC) Foundation
ID : PROSPEcT research project, www.crispr-plants.unito.it
Organisme : Cassa di Risparmio di Cuneo (CRC) Foundation
ID : PROSPEcT research project, www.crispr-plants.unito.it
Informations de copyright
© 2024. The Author(s).
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