Evaluation of the antibacterial and antifungal properties of oleuropein, olea Europea leaf extract, and thymus vulgaris oil.
Thymus Plant
/ chemistry
Iridoid Glucosides
/ pharmacology
Olea
/ chemistry
Plant Extracts
/ pharmacology
Antifungal Agents
/ pharmacology
Plant Leaves
Anti-Bacterial Agents
/ pharmacology
Iridoids
/ pharmacology
Microbial Sensitivity Tests
Aspergillus niger
/ drug effects
Candida albicans
/ drug effects
Plant Oils
/ pharmacology
Pseudomonas aeruginosa
/ drug effects
Staphylococcus aureus
/ drug effects
Escherichia coli
/ drug effects
Antimicrobial
Oleuropein
Olive leaf extracts
Thyme
Journal
BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232
Informations de publication
Date de publication:
09 Aug 2024
09 Aug 2024
Historique:
received:
27
02
2024
accepted:
18
07
2024
medline:
10
8
2024
pubmed:
10
8
2024
entrez:
9
8
2024
Statut:
epublish
Résumé
Although synthetic preservatives and antioxidants may have high antimicrobial and antioxidant activity, they are usually associated with adverse effects on human health. Currently, there is a growing interest in natural antimicrobial and antioxidant agents. This study aimed to evaluate the antimicrobial activity of two medicinal plant extracts and one active compound. Olive leaf extracts (0.2, 0.3, and 0.4% w/v), oleuropein (0.2, 0.4, and 0.6% w/v), thyme oil (0.1%), and oleuropein in combination with thyme oil (0.4% w/v and 0.1% v/v) were used against three bacterial strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) and two fungal strains (Candida albicans and Aspergillus niger). The use of oleuropein resulted in complete antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. In this context, a reduction of 7 logs was achieved during the storage period (4 weeks). Oleuropein showed no fungal activity at low concentrations (0.2%), but Aspergillus niger was reduced by 2.35 logs at higher concentrations (0.6% w/v). Similar antibacterial and antifungal properties were observed for the olive leaf extracts. Oleuropein at a concentration of 0.4 w/v and a mixture of oleuropein and thyme at concentrations of 0.4 and 0.1 (v/v) showed strong antimicrobial activity against the studied microorganisms. Olive leaf extract, thyme oil, and oleuropein have strong antibacterial and weak antifungal properties. There was a good synergistic effect between oleuropein and thymol.
Sections du résumé
BACKGROUND
BACKGROUND
Although synthetic preservatives and antioxidants may have high antimicrobial and antioxidant activity, they are usually associated with adverse effects on human health. Currently, there is a growing interest in natural antimicrobial and antioxidant agents. This study aimed to evaluate the antimicrobial activity of two medicinal plant extracts and one active compound. Olive leaf extracts (0.2, 0.3, and 0.4% w/v), oleuropein (0.2, 0.4, and 0.6% w/v), thyme oil (0.1%), and oleuropein in combination with thyme oil (0.4% w/v and 0.1% v/v) were used against three bacterial strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) and two fungal strains (Candida albicans and Aspergillus niger).
RESULTS
RESULTS
The use of oleuropein resulted in complete antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. In this context, a reduction of 7 logs was achieved during the storage period (4 weeks). Oleuropein showed no fungal activity at low concentrations (0.2%), but Aspergillus niger was reduced by 2.35 logs at higher concentrations (0.6% w/v). Similar antibacterial and antifungal properties were observed for the olive leaf extracts. Oleuropein at a concentration of 0.4 w/v and a mixture of oleuropein and thyme at concentrations of 0.4 and 0.1 (v/v) showed strong antimicrobial activity against the studied microorganisms.
CONCLUSION
CONCLUSIONS
Olive leaf extract, thyme oil, and oleuropein have strong antibacterial and weak antifungal properties. There was a good synergistic effect between oleuropein and thymol.
Identifiants
pubmed: 39123180
doi: 10.1186/s12906-024-04596-x
pii: 10.1186/s12906-024-04596-x
doi:
Substances chimiques
oleuropein
2O4553545L
Iridoid Glucosides
0
Plant Extracts
0
Antifungal Agents
0
Anti-Bacterial Agents
0
Iridoids
0
Plant Oils
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
297Informations de copyright
© 2024. The Author(s).
Références
Alwafa RA, Mudalal S, Mauriello G. Origanum syriacum L. (Za’atar), from raw to Go: a review. Plants. 2021;10:1001.
Zaazaa A, Mudalal S, Alzuheir I, Samara M, Jalboush N, Fayyad A, Petracci M. The impact of Thyme and Oregano essential oils dietary supplementation on Broiler Health, growth performance, and prevalence of growth-related breast muscle abnormalities. Animals. 2022;12:3065.
pubmed: 36359189
pmcid: 9653697
doi: 10.3390/ani12213065
Mudalal S, Zaazaa A, Omar JA. Effects of medicinal plants extract with antibiotic free diets on broilers growth performance and incidence of muscles abnormalities. Braz J Poult Sci. 2021;23:001–8.
doi: 10.1590/1806-9061-2020-1342
Thangavelu R, Sundararaju P, Sathiamoorthy S. Management of anthracnose disease of banana caused by Colletotrichum musae using plant extracts. J Hortic Sci Biotechnol. 2004;79:664–68.
doi: 10.1080/14620316.2004.11511823
Alali FQ, Tawaha K, El-Elimat T, Syouf M, El-Fayad M, Abulaila K, Nielsen SJ, Wheaton WD, Falkinham JO, Oberlies NH. Antioxidant activity and total phenolic content of aqueous and methanolic extracts of Jordanian plants. Nat Prod Res. 2017;21:1121–31.
doi: 10.1080/14786410701590285
Moreira MR, Ponce AG, del Valle CE, Roura SI. Inhibitory parameters of essential oils to reduce a Foodborne Pathogen. LWT Food Sci Technol. 2005;38:565–70.
doi: 10.1016/j.lwt.2004.07.012
Pooley R, Peterson L. Mechanisms of microbial susceptibility and resistance to antimicrobial agents. In: Shulman ST, Phair JP, Peterson LR, John R, Warren, editors. The biologic and clinical basis of Infectious diseases. 5th ed. J,R.; Philadelphia: W.B. Saunders Company; 1997. p. 550.
Somova LI, Shode FO, Ramnanan P, Nadar A. Antihypertensive, antiatherosclerotic and antioxidant activity of tripenoids isolated from Olea europaea. Subspecies Africana Leaves J Ethnopharmacol. 2003;84:299–305.
pubmed: 12648829
doi: 10.1016/S0378-8741(02)00332-X
Benavente-Garcia O, Castillo J, Lorente J, Ortuno A, Del-Rio JA. Antioxidant activity of phenolics from Olea europaea L. leaves. Food Chem. 2000;49(4):2480–5.
Tutour BL, Guedon D. Antioxidative activities of Olea Europea leaves and related phenolic compounds. Phytochemistry. 1992;31(4):1173–8.
doi: 10.1016/0031-9422(92)80255-D
Visioli F, Galli C. Olives and their production waste products as sources of bioactive compounds. Curr Top Nutraceutical Res. 2003;1:85–8.
Owen RW, Giacosa G, Hull WE, Haubner R, Wurtele G, Spiegelhalder B, Bartsch H. Olive oil consumption and health: the possible role of antioxidants. Lancet Oncol. 2000;1(2):107–12.
pubmed: 11905662
doi: 10.1016/S1470-2045(00)00015-2
Basch E, Ulbricht V, Hammerness P, Bevins A, Sollars D. Thyme (Thymus vulgarisl) thymol. J Herb Pharmacother. 2004;4:63–78.
pubmed: 15829470
Imelouane B, Hassa A, Wathelet IP, Ankit M, Khedid K, El-Bachiri AA. Chemical composition and antimicrobial activity of essential oil of thyme (Thymus Vulgaris) from eastern Morocco. Int J Agric Biol. 2009;11:205–8.
Grigore A, Paraschiv I, Mihul A, Corina B, Draghici EM, Ichim M. Chemical composition and antioxidant activity of Thymus vulgaris L. volatile oil obtained by two different methods. Rom Biotechnol Lett. 2010;15:5436–43.
Al Maqtari M, Alghalibi S, Alhamzy E. Chemical composition and antimicrobial activity of essential oil of Thymus vulgaris from Yemen. Turk J Biochem. 2011;36:342–9.
Aliabadi MA, Darsanaki RK, Rokhi ML, Nourbakhsh M, Raeisi G. Antimicrobial activity of olive leaf aqueous extract. Ann Biol Res. 2012;3:4189–91.
Al-Rimawi F, Sbeih M, Amayreh M, Rahhal B, Mudalal S. Evaluation of the effectiveness of natural extract as a substituent for synthetic preservatives and antioxidants in pharmaceutical preparations. Saudi Pharm J. 2024;32(5). https://doi.org/10.1016/j.jsps.2024.102014 .
Zorić N, Kosalec I. The Antimicrobial activities of Oleuropein and Hydroxytyrosol. In: Rai M, Kosalec I, editors. Promising antimicrobials from Natural products. Cham: Springer; 2022. https://doi.org/10.1007/978-3-030-83504-0_5 .
doi: 10.1007/978-3-030-83504-0_5
Salma NM. Antibacterial activity of Olive (Olea europaea) leaves and Arugula (Eruca sativa) seeds extract. Int J Pharmacogn Phytochem. 2015;7:307–10.
Markin D, Duek L, Berdicevsky I. In vitro antimicrobial activity of olive leaves. Mycoses. 2003;46:132–1366.
pubmed: 12870202
doi: 10.1046/j.1439-0507.2003.00859.x
Battinelli L, Daniele C, Cristiani G, Mazzanti G. In vitro antifungal and anti-elastase activity of some aliphatic aldehydes from Olea europaea L. fruit. Phytomedicine. 2006;13:558–63.
pubmed: 16920510
doi: 10.1016/j.phymed.2005.09.009
KarygianniL, Cecere M, Skaltsounis AL, Argyropoulou A, Hellwig E, Aligiannis N, Wittmer A, Al-Ahmad A. High-level Antimicrobial Efficacy of Representative Mediterranean Natural Plant extracts against oral microorganisms. Biomed Res Int 2014; Article ID 839019, 8 pages.
Gumgumjee NM, Hajar AS. Antimicrobial activities and phytochemical properties of Saudi oleaeuropaea subsp cuspidate. Life Sci J. 2014;11:232–7.
Gokmen M, Kara R, Akkaya L, Torlak E, Onen A. Evaluation of antimicrobial activity in olive (olea europaea) leaf extract. Am J Microbiol. 2014;5:37–40.
Korukluoglu M, Sahan Y, Yigit A. Antifungal properties of olive leaf extracts and their phenolic compounds. J Food Saf. 2008;28:76–87.
doi: 10.1111/j.1745-4565.2007.00096.x
Pereira AP, Ferreira ICFR, Marcelino F, Valentao P, Andrade PB, Seabra R, Estevinho L, Bento A, Pereira JA. Phenolic compounds and Antimicrobial Activity of Olive (Olea europaea L. Cv Cobrancosa) Leaves Molecules. 2007;12:1153–62.
pubmed: 17873849
Erdohan O, Turhan KN. Olive leaf extract and usage for development of antimicrobial food packaging, Science against microbial pathogens: communicating current research and technological advances. Publisher: Formatex, ; 2012. pp. 1094–101. Department of Food Engineering, University of Mersin, 33343, Çiftlikköy, Mersin, Turkey.
Ilias F, Kholkhal W, Gaouar N, Bekhechi C, Bekkara FA. Antibacterial and antifungal activities of olive (Olea europaea L.) from Algeria. J Microbiol Biotechnol res. 2011;1:69–73.
Hussain A, Qarshi QA, Liaqat R, Akhtar S, Aziz I, Ullah I, Shinwari ZK. Antimicrobial potential of leaf and fruit extracts and oils of wild and cultivate dedible olive. Pak J Bot. 2014;46:1463–8.
Dada AA, Ifesan BOT, Fashakin JF. Antimicrobial and antioxidant properties of selected local spices used in Kunun Beverage in Nigeria. Acta Sci Pol Technol Aliment. 2013;12:373–8.
Antunes BDF, Otero DM, Oliveira FM, Jacques AC, Gandra EA, Zambiazi RC. Antioxidant and antimicrobial activity of olive trees cultivated in the Campanha Gaúcha region. Braz J Dev. 2020;6:21791–805.
doi: 10.34117/bjdv6n4-374
Liu Y, McKeever LC, Malik NSA. Assessment of the antimicrobial activity of Olive Leaf Extract against Foodborne bacterial pathogens. Front Microbiol. 2017;8:113.
pubmed: 28210244
pmcid: 5288333
Rafiei Z, Jafari SM, Alami M, Khomeiri M. Evaluation of antimicrobial activity of olive leaf extracts by ELISA method. IJMAPR. 2012;28:280–92.
Korukluoglu M, Sahan Y, Yigit A, Karakas R. Antifungal activity of olive leaf (Olea Europaea L.) extracts from the Trilye Region of Turkey. Ann Microbiol. 2006;56:359–62.
doi: 10.1007/BF03175032
Korukluoglu M, Sahan Y, Yigit A, Ozer ET, Gucer S. Antibacterial activity and chemical constitution so Olea europaea L. leaf extracts. J Food Process Preserv. 2010;34:383–96.
doi: 10.1111/j.1745-4549.2008.00318.x
Borjan D, Leitgeb M, Knez Z, Hrncic MK. Microbiological and antioxidant activity of Phenolic compounds in Olive Leaf Extract. Molecules. 2020;25:5946.
pubmed: 33334001
pmcid: 7765412
doi: 10.3390/molecules25245946
Simat V, Skroza D, Tabanelli G, Cagalj, Pasini F, Gomez-Caravaca AM, Fernandez-Fernandez C, Sternisa M, Mozina SS, Ozogul Y, Mekinic IG. Antioxidant and antimicrobial activity of Hydroethanolic Leaf extracts from six Mediterranean Olive cultivars. Antioxidants. 2022;11(9):1656.
pubmed: 36139730
pmcid: 9495989
doi: 10.3390/antiox11091656
Nasrollahi Z, Abolhasannezhad M. Evaluation of the antifungal activity of olive leaf aqueous extracts against Candida albicans PTCC-5027. Cur Med Mycol. 2015;1(4):37–9.
doi: 10.18869/acadpub.cmm.1.4.37
Giacometti J, Milovanovic S, Momcilovic MJ, Bubonja-Sonje M. Evaluation of antioxidant activity of olive leaf extract obtained by ultrasound-assisted extraction and their antimicrobial activity against bacterial pathogens from food. Int J Food Sci Technol. 2021;56:4843–50.
doi: 10.1111/ijfs.15319
Guo L, Gong S, Wang Y, Sun Q, Duo K, Fei P. Antibacterial Activity of Olive Oil Polyphenol Extract against Salmonella Typhimurium and Staphylococcus aureus: possible mechanisms. Foodborne Pathog Dis. 2020;17(6):396–403.
pubmed: 31755743
doi: 10.1089/fpd.2019.2713
Marino M, Bersani C, Comi G. Antimicrobial activity of the essential oils of Thymus vulgaris L. measured using a bioimpedometric method. J Food Prot. 1999;62(9):1017–23.
pubmed: 10492476
doi: 10.4315/0362-028X-62.9.1017
Boskovica M, Zdravkovic N, Ivanovic J, Janjic J, Djordjevic J, Starcevic M, Baltic MZ. Antimicrobial activity of Thyme (Tymus Vulgaris) and oregano (Origanum vulgare) essential oils against some food-borne microorganisms. Procedia Food Sci. 2015;5:18–21.
doi: 10.1016/j.profoo.2015.09.005
Cetin B, Cakmakci S, Cakmaci R. The investigation of antimicrobial activity of thyme and oregano essential oils. Turk J Agric for. 2011;35(2):145–54.
Gavaric N, Mozina SS, Kladar N, Bozin B. Chemical profile, antioxidant and antibacterial activity of thyme and oregano essential oils, thymol and carvacrol and their possible synergism. J Essent Oil Bear Plants. 2015;18(4):1013–21.
doi: 10.1080/0972060X.2014.971069