Epizootic Hemorrhagic Disease Agar Gel Immunodiffusion Assay.
Agar gel
Bluetongue
Cross-reactivity
Epizootic hemorrhagic disease
Immunodiffusion
Precipitation line
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
10
8
2024
pubmed:
10
8
2024
entrez:
10
8
2024
Statut:
ppublish
Résumé
Agar gel immunodiffusion assay (AGID) is a laboratory test which detects specific antigen-antibody interactions by the development of visible precipitation lines in a semisolid matrix. Here we describe the preparation of agar gel plates, the method to test serum samples by AGID for the presence of EHDV antibodies, and the interpretation of test results. This test has known cross-reactivity to bluetongue antibodies; therefore positive samples by this assay require additional confirmatory testing; generally, its use should be limited to healthy animal attestations where required.
Identifiants
pubmed: 39126628
doi: 10.1007/978-1-0716-4035-7_8
doi:
Substances chimiques
Agar
9002-18-0
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
137-143Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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