Functional plasticity of HCO3- uptake and CO2 fixation in Cupriavidus necator H16.

Biomanufacturing C1 metabolism CO(2) conversion CRAGE Carbonic anhydrase DAB2 Gas fermentation Genome engineering Rubisco

Journal

Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523

Informations de publication

Date de publication:
08 Aug 2024
Historique:
received: 08 05 2024
revised: 01 08 2024
accepted: 01 08 2024
medline: 11 8 2024
pubmed: 11 8 2024
entrez: 10 8 2024
Statut: aheadofprint

Résumé

Despite its prominence, the ability to engineer Cupriavidus necator H16 for inorganic carbon uptake and fixation is underexplored. We tested the roles of endogenous and heterologous genes on C. necator inorganic carbon metabolism. Deletion of β-carbonic anhydrase can had the most deleterious effect on C. necator autotrophic growth. Replacement of this native uptake system with several classes of dissolved inorganic carbon (DIC) transporters from Cyanobacteria and chemolithoautotrophic bacteria recovered autotrophic growth and supported higher cell densities compared to wild-type (WT) C. necator in batch culture. Strains expressing Halothiobacillus neopolitanus DAB2 (hnDAB2) and diverse rubisco homologs grew in CO

Identifiants

DOI: 10.1016/j.biortech.2024.131214 PMID: 39127361
pubmed: 39127361
pii: S0960-8524(24)00918-0
doi: 10.1016/j.biortech.2024.131214
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

131214

Informations de copyright

Copyright © 2024. Published by Elsevier Ltd.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Justin Panich (J)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Emili Toppari (E)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Sara Tejedor-Sanz (S)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Advanced Biofuel and Bioproducts Process Development Unit, Lawrence Berkeley NationalLaboratory, Emeryville, CA 94608, USA.

Bonnie Fong (B)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Eli Dugan (E)

Department of Molecular and Cell Biology, University of California-Berkeley, Berkeley, CA, 94720, USA.

Yan Chen (Y)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Christopher J Petzold (CJ)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Zhiying Zhao (Z)

The US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, CA 94720, USA.

Yasuo Yoshikuni (Y)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; The US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, CA 94720, USA; Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, CA94720, USA.

David F Savage (DF)

Department of Molecular and Cell Biology, University of California-Berkeley, Berkeley, CA, 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.

Steven W Singer (SW)

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: justinpanich@lbl.gov.

Classifications MeSH