Exploring the effects of three-finger toxins from Naja ashei venom on neuronal and immunological cancer cell membranes.
Langmuir monolayer
Lipid
Membrane
Three-finger protein
Toxins
Venom
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 Aug 2024
10 Aug 2024
Historique:
received:
10
04
2024
accepted:
05
08
2024
medline:
11
8
2024
pubmed:
11
8
2024
entrez:
10
8
2024
Statut:
epublish
Résumé
Three-finger proteins are the most abundant toxins in the venom of Naja ashei, a snake species from the Elapidae family. This research aimed to describe the effects of varying charges of these proteins, isolated from Naja ashei venom using SEC and IEX chromatography. The study examined how differently charged three-finger toxin fractions interact with and affect neuroblastoma (SK-N-SH) and promyeloblast (HL-60) cells, as well as model Langmuir membranes and liposomes designed to mimic cellular lipid composition. Findings revealed that protein surface charges significantly impact cell survival (MTT assay), membrane damage (lactate dehydrogenase release, malondialdehyde formation), and the structural and electrochemical properties of model membranes (Langmuir membranes and zeta potential for liposomes and cancer cell lines). Results indicated that SK-N-SH cells, characterized by a higher negative charge on their cell membranes, interacted more effectively with positively charged toxins than HL-60 cells. However, the mechanism of these electrostatic interactions is complex. The research demonstrated that electrostatic and mechanical membrane modifications induced by venom proteins can significantly affect cell metabolism. Additionally, the total charge of the membrane, influenced by polar lipid components and phospholipid saturation, plays a decisive role in toxin interaction.
Identifiants
pubmed: 39127758
doi: 10.1038/s41598-024-69459-4
pii: 10.1038/s41598-024-69459-4
doi:
Substances chimiques
Elapid Venoms
0
Liposomes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18570Subventions
Organisme : Slovak Research and Development Agency, Ministry of Education, Science, Research and Sport of the Slovak Republic
ID : APVV-17-0017
Informations de copyright
© 2024. The Author(s).
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