Protocol for generation and engineering of thyroid cell lineages using CRISPR-Cas9 editing to recapitulate thyroid cancer histotype progression.

CRISPR Cancer Cell Differentiation Cell culture

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
10 Aug 2024
Historique:
received: 07 03 2024
revised: 23 05 2024
accepted: 25 07 2024
medline: 11 8 2024
pubmed: 11 8 2024
entrez: 11 8 2024
Statut: aheadofprint

Résumé

Thyroid carcinoma represents the first malignancy among the endocrine organs. Investigating the cellular hierarchy and the mechanisms underlying the initiation of thyroid carcinoma is crucial in thyroid cancer research. Here, we present a protocol for deriving thyroid cell lineage from human embryonic stem cells. We also describe steps for engineering thyroid progenitor cells utilizing CRISPR-Cas9 technology, which can be used to perform in vivo studies, thus facilitating the development of representative thyroid tumorigenesis models. For complete details on the use and execution of this protocol, please refer to Veschi et al.

Identifiants

DOI: 10.1016/j.xpro.2024.103263 PMID: 39128010
pubmed: 39128010
pii: S2666-1667(24)00428-3
doi: 10.1016/j.xpro.2024.103263
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103263

Informations de copyright

Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Vincenzo Davide Pantina (VD)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy.

Francesco Verona (F)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy.

Alice Turdo (A)

Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, 90100 Palermo, Italy.

Veronica Veschi (V)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy; Department of Molecular Medicine, University La Sapienza, 00161 Rome, Italy.

Chiara Modica (C)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy.

Melania Lo Iacono (M)

Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, 90100 Palermo, Italy.

Miriam Gaggianesi (M)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy.

Sebastiano Di Bella (S)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy.

Matilde Todaro (M)

Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, 90100 Palermo, Italy; A.O.U.P. "Paolo Giaccone", University of Palermo, 90100 Palermo, Italy.

Simone Di Franco (S)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy. Electronic address: simone.difranco@unipa.it.

Giorgio Stassi (G)

Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy. Electronic address: giorgio.stassi@unipa.it.

Classifications MeSH