Determination of metal ion transport rate of human ZIP4 using stable zinc isotopes.

ZIP kinetics stable isotope transport assay turnover number zinc transporter

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
09 Aug 2024
Historique:
received: 06 01 2024
revised: 28 07 2024
accepted: 01 08 2024
medline: 12 8 2024
pubmed: 12 8 2024
entrez: 11 8 2024
Statut: aheadofprint

Résumé

The essential microelement zinc is absorbed in the small intestine mainly by the zinc transporter ZIP4, a representative member of the Zrt/Irt-like protein (ZIP) family. ZIP4 is reportedly upregulated in many cancers, making it a promising oncology drug target. To date, there have been no reports on the turnover number of ZIP4, which is a crucial missing piece of information needed to better understand the transport mechanism. In this work, we used a non-radioactive zinc isotope,

Identifiants

DOI: 10.1016/j.jbc.2024.107661 PMID: 39128710
pubmed: 39128710
pii: S0021-9258(24)02162-8
doi: 10.1016/j.jbc.2024.107661
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

107661

Informations de copyright

Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare no conflicts of interest with the contents of this article.

Auteurs

Yuhan Jiang (Y)

Department of Chemistry, Michigan State University, East Lansing, MI, USA.

Keith MacRenaris (K)

Department of Chemistry, Michigan State University, East Lansing, MI, USA; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA; Elemental Health Institute, Michigan State University, East Lansing, MI, USA; Quantitative Bio Element Analysis and Mapping (QBEAM) Center, Michigan State University, East Lansing, MI, USA.

Thomas V O'Halloran (TV)

Department of Chemistry, Michigan State University, East Lansing, MI, USA; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA; Elemental Health Institute, Michigan State University, East Lansing, MI, USA; Quantitative Bio Element Analysis and Mapping (QBEAM) Center, Michigan State University, East Lansing, MI, USA. Electronic address: ohallor8@msu.edu.

Jian Hu (J)

Department of Chemistry, Michigan State University, East Lansing, MI, USA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA. Electronic address: hujian1@msu.edu.

Classifications MeSH