Recordings of Superior Laryngeal Nerve Sensory Nerve Action Potentials in a Rat Model.
nerve conduction studies
superior laryngeal nerve
Journal
The Laryngoscope
ISSN: 1531-4995
Titre abrégé: Laryngoscope
Pays: United States
ID NLM: 8607378
Informations de publication
Date de publication:
12 Aug 2024
12 Aug 2024
Historique:
revised:
09
05
2024
received:
16
02
2024
accepted:
21
05
2024
medline:
12
8
2024
pubmed:
12
8
2024
entrez:
12
8
2024
Statut:
aheadofprint
Résumé
Superior laryngeal nerve (SLN) function is critical to laryngeal sensation. Sensory dysfunction in the larynx, mediated through the internal branch of the superior laryngeal nerve (iSLN), is thought to occur with aging and neurodegenerative disease. However, objective analysis of iSLN neurophysiology is difficult due to its anatomic location and small diameter. This study measures sensory nerve action potentials (SNAP) from the iSLN in a rat model. SNAP data were obtained from two adult rat strains (Sprague-Dawley, SD and Fischer 344 × Brown Norway F1 Hybrid rats, FBN). Evoked responses were obtained by stimulating the main trunk of the SLN and recording the response using a 160-μm cuff electrode placed around the iSLN. SNAP were averaged from 10 stimulations. Laryngeal adductor reflex (LAR) threshold measurements were obtained with stimulation of the iSLN and direct laryngoscopy. The sections of the iSLN were obtained for histologic analysis. SLN-evoked responses were successfully obtained in 18 hemi-laryngeal preparations (SD n = 13 and FBN n = 5) with corresponding LAR threshold measurements. Mean(±SD) SNAP latency, total duration, amplitude, negative durations, and intensity were 2.28 ms (±0.56), 2.13 ms (±0.70), 879 μV (±535), and 0.69 mA (±0.25), respectively. SLN stimulation threshold to elicit an LAR was of 0.84 mA (±0.31). It is feasible to record evoked SLN responses in two adult rat strains. This work may lead to a tractable animal model for objective measurements of SLN neurophysiology with various disease states. N/A Laryngoscope, 2024.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : American Laryngological, Rhinological and Otological Society
ID : Triological Society 2021 Career Development Award
Informations de copyright
© 2024 The American Laryngological, Rhinological and Otological Society, Inc.
Références
Santoso LF, Jafari S, Kim DY, Paydarfar D. The internal superior laryngeal nerve in humans: evidence for pure sensory function. Laryngoscope. 2021;131(1):E207‐E211. https://doi.org/10.1002/LARY.28642.
Sulica L. The superior laryngeal nerve: function and dysfunction. Otolaryngol Clin North Am. 2004;37(1):183‐201. https://doi.org/10.1016/S0030-6665(03)00175-0.
Teitelbaum BJ, Wenig BL. Superior laryngeal nerve injury from thyroid surgery. Head Neck. 1995;17(1):36‐40. https://doi.org/10.1002/HED.2880170108.
Aviv JE. Effects of aging on sensitivity of the pharyngeal and supraglottic areas. Am J Med. 1997;103(5A):74S‐76S. https://doi.org/10.1016/S0002-9343(97)00327-6.
Halum SL, Sycamore DL, McRae BR. A new treatment option for laryngeal sensory neuropathy. Laryngoscope. 2009;119(9):1844‐1847. https://doi.org/10.1002/LARY.20553.
Aslam M, Vaezi MF. Dysphagia in the elderly. Gastroenterol Hepatol (NY). 2013;9(12):784.
Santos DAR, Arcanjo DPS, de Melo Silva GMB, et al. Factors associated with aspiration pneumonia and preventive measures in hospitalized elderly: a systematic review of observational studies. Rev CEFAC. 2020;22(6):2920. https://doi.org/10.1590/1982-0216/20202262920.
Bolton CF, Carter KM. Human sensory nerve compound action potential amplitude: variation with sex and finger circumference. J Neurol Neurosurg Psychiatry. 1980;43(10):925‐928. https://doi.org/10.1136/JNNP.43.10.925.
Malessy MJA, Pondaag W, Van Dijk JG. Electromyography, nerve action potential, and compound motor action potentials in obstetric brachial plexus lesions: validation in the absence of a “gold standard.”. Neurosurgery. 2009;65(4 Suppl):A153‐A159. https://doi.org/10.1227/01.NEU.0000338429.66249.7D.
Cvancara DJ, de Leon JA, Baertsch HC, et al. Neurophysiology of the superior laryngeal nerve in an in vivo rat model. Laryngoscope. 2023;134:1778‐1784. https://doi.org/10.1002/LARY.31087.
Flint PW, Downs DH, Coltrera MD. Laryngeal synkinesis following reinnervation in the rat. Neuroanatomic and physiologic study using retrograde fluorescent tracers and electromyography. Ann Otol Rhinol Laryngol. 1991;100(10):797‐806. https://doi.org/10.1177/000348949110001003.
Riede T. Subglottal pressure, tracheal airflow, and intrinsic laryngeal muscle activity during rat ultrasound vocalization. J Neurophysiol. 2011;106(5):2580‐2592. https://doi.org/10.1152/JN.00478.2011.
Alli O, Berzofsky C, Sharma S, Pitman MJ. Development of the rat larynx: a histological study. Laryngoscope. 2013;123(12):3093‐3098. https://doi.org/10.1002/LARY.24145.
Weissbrod P, Pitman MJ, Sharma S, Bender A, Schaefer SD. Quantity and three‐dimensional position of the recurrent and superior laryngeal nerve lower motor neurons in a rat model. Ann Otol Rhinol Laryngol. 2011;120(11):761‐768. https://doi.org/10.1177/000348941112001111.
Hernández‐Morato I, Pascual‐Font A, Ramírez C, et al. Somatotopy of the neurons innervating the cricothyroid, posterior cricoarytenoid, and thyroarytenoid muscles of the rat's larynx. Anat Rec (Hoboken). 2013;296(3):470‐479. https://doi.org/10.1002/AR.22643.
Suckow MA, Weisbroth SH, Franklin CL. The Laboratory Rat, 2nd edn. Laboratory rat 2005:1–912. https://doi.org/10.1016/B978-0-12-074903-4.X5000-6
Lipman RD, Chrisp CE, Hazzard DWG, Bronson RT. Pathologic characterization of brown Norway, brown Norway x Fischer 344, and Fischer 344 x brown Norway rats with relation to age. J Gerontol A Biol Sci Med Sci. 1996;51(1):B54‐B59. https://doi.org/10.1093/GERONA/51A.1.B54.
Tatlipinar A, Güneş P, Özbeyli D, Çimen B, Gökçeer T. Effects of ovariectomy and estrogen replacement therapy on laryngeal tissue: a histopathological experimental animal study. Otolaryngol – Head Neck Surg. 2011;145(6):987‐991. https://doi.org/10.1177/0194599811423638/ASSET/IMAGES/LARGE/10.1177_0194599811423638-FIG1.JPEG.
Moore CL, White RH. Sex differences in sensory and motor branches of the pudendal nerve of the rat. Horm Behav. 1996;30(4):590‐599. https://doi.org/10.1006/HBEH.1996.0062.
Baertsch HC, Cvancara D, Bhatt NK. Utilizing novel recurrent laryngeal motor nerve conduction studies to characterize the aging larynx: a pilot study. Laryngoscope Invest Otolaryngol. 2023;8(3):739‐745. https://doi.org/10.1002/LIO2.1071.
Berke GS, Moore DM, Gerratt BR, Hanson DG, Natividad M. Effect of superior laryngeal nerve stimulation on phonation in an in vivo canine model. Am J Otolaryngol. 1989;10(3):181‐187. https://doi.org/10.1016/0196-0709(89)90060-4.
Zaimi A, Wabartha M, Herman V, Antonsanti PL, Perone CS, Cohen‐Adad J. AxonDeepSeg: automatic axon and myelin segmentation from microscopy data using convolutional neural networks. Sci Rep. 2018;8(1):3816. https://doi.org/10.1038/S41598-018-22181-4.
Wong AL, Hricz N, Malapati H, et al. A simple and robust method for automating analysis of naïve and regenerating peripheral nerves. PLoS One. 2021;16(7):e0248323. https://doi.org/10.1371/JOURNAL.PONE.0248323.
Tiago R, Pontes P, do Brasil OC. Age‐related changes in human laryngeal nerves. Otolaryngol Head Neck Surg. 2007;136(5):747‐751. https://doi.org/10.1016/J.OTOHNS.2006.11.054.
Baertsch H, Cvancara DJ, Paniello RC, Hillel AD, Bhatt NK. Recurrent laryngeal motor nerve conduction studies in a rat model: establishing an objective measure for investigating laryngeal innervation. Muscle Nerve. 2023;68(4):471‐475. https://doi.org/10.1002/MUS.27932.
Leandri M, Saturno M, Cilli M, Bisaglia M, Lunardi G. Compound action potential of sensory tail nerves in the rat. Exp Neurol. 2007;203(1):148‐157. https://doi.org/10.1016/J.EXPNEUROL.2006.08.001.