Measuring Retrograde Actin Flow in Neuronal Growth Cones.

Animals Growth Cones / metabolism Actins / metabolism Aplysia / metabolism Actin Cytoskeleton / metabolism Neurons / metabolism Microscopy, Fluorescence / methods Cells, Cultured Kymography / methods Time-Lapse Imaging / methods
Actin cytoskeleton Kymograph Neuronal growth cone Retrograde F-actin flow Time-lapse imaging

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2024
Historique:
medline: 13 8 2024
pubmed: 13 8 2024
entrez: 12 8 2024
Statut: ppublish

Résumé

Actin flow refers to the motion of the F-actin cytoskeleton and has been observed in many different cell types, especially in motile cells including neuronal growth cones. The direction of the actin flow is generally retrograde from the periphery toward the center of the cell. Actin flow can be harnessed for forward movement of the cell through substrate-cytoskeletal coupling; thus, a key function of actin flow is in cell locomotion. In this chapter, we illustrate three different methods of quantifying retrograde F-actin flow in growth cones derived from cultured Aplysia bag cell neurons. These methods include tracking the movement of surface marker beads as well as kymograph analysis of time-lapse sequences acquired by differential interference contrast (DIC) imaging or fluorescent speckle microscopy (FSM). Due to their large size, Aplysia neuronal growth cones are uniquely suited for these methods; however, they can also be applied to any other growth cones with clear F-actin-rich peripheral domains.

Identifiants

DOI: 10.1007/978-1-0716-3969-6_18 PMID: 39134856
pubmed: 39134856
doi: 10.1007/978-1-0716-3969-6_18
doi:

Substances chimiques

Actins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

265-282

Informations de copyright

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Dent EW, Gupton SL, Gertler FB (2011) The growth cone cytoskeleton in axon outgrowth and guidance. Cold Spring Harb Perspect Biol 3(3). https://doi.org/10.1101/cshperspect.a001800
Tojima T, Hines JH, Henley JR, Kamiguchi H (2011) Second messengers and membrane trafficking direct and organize growth cone steering. Nat Rev Neurosci 12(4):191–203. https://doi.org/10.1038/nrn2996
doi: 10.1038/nrn2996 pubmed: 21386859 pmcid: 3133775
Zang Y, Chaudhari K, Bashaw GJ (2021) New insights into the molecular mechanisms of axon guidance receptor regulation and signaling. Curr Top Dev Biol 142:147–196. https://doi.org/10.1016/bs.ctdb.2020.11.008
doi: 10.1016/bs.ctdb.2020.11.008 pubmed: 33706917 pmcid: 8456978
Cajal R (1890) A quelle époque apparaissent les expansions des cellules nerveuses de la moelle épinière du poulet. Anatomischer Anzeiger 5:609–613
Munoz-Lasso DC, Roma-Mateo C, Pallardo FV, Gonzalez-Cabo P (2020) Much more than a scaffold: cytoskeletal proteins in neurological disorders. Cells 9(2). https://doi.org/10.3390/cells9020358
Blanquie O, Bradke F (2018) Cytoskeleton dynamics in axon regeneration. Curr Opin Neurobiol 51:60–69. https://doi.org/10.1016/j.conb.2018.02.024
doi: 10.1016/j.conb.2018.02.024 pubmed: 29544200
He Y, Ren Y, Wu B, Decourt B, Lee AC, Taylor A, Suter DM (2015) Src and cortactin promote lamellipodia protrusion and filopodia formation and stability in growth cones. Mol Biol Cell 26(18):3229–3244. https://doi.org/10.1091/mbc.E15-03-0142
doi: 10.1091/mbc.E15-03-0142 pubmed: 26224308 pmcid: 4569314
Lee AC, Suter DM (2008) Quantitative analysis of microtubule dynamics during adhesion-mediated growth cone guidance. Dev Neurobiol 68(12):1363–1377. https://doi.org/10.1002/dneu.20662
doi: 10.1002/dneu.20662 pubmed: 18698606 pmcid: 2745986
Athamneh AI, Cartagena-Rivera AX, Raman A, Suter DM (2015) Substrate deformation predicts neuronal growth cone advance. Biophys J 109(7):1358–1371. https://doi.org/10.1016/j.bpj.2015.08.013
doi: 10.1016/j.bpj.2015.08.013 pubmed: 26445437 pmcid: 4601092
Suter DM, Errante LD, Belotserkovsky V, Forscher P (1998) The Ig superfamily cell adhesion molecule, apCAM, mediates growth cone steering by substrate-cytoskeletal coupling. J Cell Biol 141(1):227–240. https://doi.org/10.1083/jcb.141.1.227
doi: 10.1083/jcb.141.1.227 pubmed: 9531561 pmcid: 2132711
Forscher P, Smith SJ (1988) Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone. J Cell Biol 107(4):1505–1516. https://doi.org/10.1083/jcb.107.4.1505
doi: 10.1083/jcb.107.4.1505 pubmed: 3170637
Schaefer AW, Kabir N, Forscher P (2002) Filopodia and actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cones. J Cell Biol 158(1):139–152. https://doi.org/10.1083/jcb.200203038
doi: 10.1083/jcb.200203038 pubmed: 12105186 pmcid: 2173029
Tanaka EM, Kirschner MW (1991) Microtubule behavior in the growth cones of living neurons during axon elongation. J Cell Biol 115(2):345–363. https://doi.org/10.1083/jcb.115.2.345
doi: 10.1083/jcb.115.2.345 pubmed: 1918145
Tanaka E, Sabry J (1995) Making the connection: cytoskeletal rearrangements during growth cone guidance. Cell 83(2):171–176. https://doi.org/10.1016/0092-8674(95)90158-2
doi: 10.1016/0092-8674(95)90158-2 pubmed: 7585934
Miller KE, Suter DM (2018) An integrated cytoskeletal model of neurite outgrowth. Front Cell Neurosci 12:447. https://doi.org/10.3389/fncel.2018.00447
doi: 10.3389/fncel.2018.00447 pubmed: 30534055 pmcid: 6275320
Rochlin MW, Dailey ME, Bridgman PC (1999) Polymerizing microtubules activate site-directed F-actin assembly in nerve growth cones. Mol Biol Cell 10(7):2309–2327. https://doi.org/10.1091/mbc.10.7.2309
doi: 10.1091/mbc.10.7.2309 pubmed: 10397767 pmcid: 25445
Mallavarapu A, Mitchison T (1999) Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction. J Cell Biol 146(5):1097–1106. https://doi.org/10.1083/jcb.146.5.1097
doi: 10.1083/jcb.146.5.1097 pubmed: 10477762 pmcid: 2169471
Medeiros NA, Burnette DT, Forscher P (2006) Myosin II functions in actin-bundle turnover in neuronal growth cones. Nat Cell Biol 8(3):215–226. https://doi.org/10.1038/ncb1367
doi: 10.1038/ncb1367 pubmed: 16501565
Marsick BM, Flynn KC, Santiago-Medina M, Bamburg JR, Letourneau PC (2010) Activation of ADF/cofilin mediates attractive growth cone turning toward nerve growth factor and netrin-1. Dev Neurobiol 70(8):565–588. https://doi.org/10.1002/dneu.20800
doi: 10.1002/dneu.20800 pubmed: 20506164 pmcid: 2908028
Suter DM (2011) Live cell imaging of neuronal growth cone motility and guidance in vitro. Methods Mol Biol 769:65–86. https://doi.org/10.1007/978-1-61779-207-6_6
doi: 10.1007/978-1-61779-207-6_6 pubmed: 21748670 pmcid: 3732825
Kandel ER (1976) Cellular basis of behavior. 727 pages.
Ren Y, Suter DM (2016) Increase in growth cone size correlates with decrease in neurite growth rate. Neural Plast 2016:3497901. https://doi.org/10.1155/2016/3497901
doi: 10.1155/2016/3497901 pubmed: 27274874 pmcid: 4870373
Suter DM, Forscher P (2001) Transmission of growth cone traction force through apCAM-cytoskeletal linkages is regulated by Src family tyrosine kinase activity. J Cell Biol 155(3):427–438. https://doi.org/10.1083/jcb.200107063
doi: 10.1083/jcb.200107063 pubmed: 11673478 pmcid: 2150837
Waterman-Storer C (2002) Fluorescent speckle microscopy (FSM) of microtubules and actin in living cells. Curr Protoc Cell Biol Chapter 4:Unit 4 10. https://doi.org/10.1002/0471143030.cb0410s13

Auteurs

Laura Pulido Cifuentes (L)

Department of Biological Sciences, Purdue University, West Lafayette, IN, USA.

Daniel M Suter (DM)

Department of Biological Sciences, Purdue University, West Lafayette, IN, USA. dsuter@purdue.edu.
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA. dsuter@purdue.edu.
Purdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, IN, USA. dsuter@purdue.edu.
Purdue Institute for Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA. dsuter@purdue.edu.
Bindley Bioscience Center, Purdue University, West Lafayette, IN, USA. dsuter@purdue.edu.
Birck Nanotechnology Center, Purdue University, West Lafayette, IN, USA. dsuter@purdue.edu.
ORCID: 0000-0002-5230-7229

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Cellules › Neurones › Neurofibres › Axones › Cônes de croissance › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Actines › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Mollusca › Gastropoda › Aplysia › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Cytosquelette d'actine › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Cellules › Neurones › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Cellules › Cellules cultivées › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Kymographie › Measuring Retrograde Actin Flow in Neuronal...
questionsmedicales.fr › Sciences de l'information › Processus de copie › Enregistrement sur magnétoscope › Imagerie accélérée › Measuring Retrograde Actin Flow in Neuronal...