Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research.

Cancer Cell Biology Molecular Biology

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
13 Aug 2024
Historique:
received: 05 04 2024
revised: 05 05 2024
accepted: 22 07 2024
medline: 14 8 2024
pubmed: 14 8 2024
entrez: 14 8 2024
Statut: aheadofprint

Résumé

Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.

Identifiants

DOI: 10.1016/j.xpro.2024.103256 PMID: 39141519
pubmed: 39141519
pii: S2666-1667(24)00421-0
doi: 10.1016/j.xpro.2024.103256
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103256

Informations de copyright

Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Anthony Casabianca (A)

Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.

Mélanie Bellina (M)

Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France.

Crissy Dudgeon (C)

Rutgers Cancer Institute of New Jersey, New Brunswick, NJ 08901, USA.

Muhammad Sadeqi Nezhad (MS)

University of Rochester Clinical and Translational Science Institute, Rochester, NY 14620, USA.

Chris Harris (C)

Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA.

Stephany Fiore (S)

Netris Pharma, 69008 Lyon. France.

Agnes Bernet (A)

Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France.

Wade Narrow (W)

Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA.

Orjola Prela (O)

Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.

Lan Wang (L)

Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.

Igor Astsaturov (I)

Department of Medical Oncology, Fox Chase Cancer Center, Philadephia, PA 19111, USA.

Patrick Mehlen (P)

Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France.

Darren R Carpizo (DR)

Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA. Electronic address: darren_carpizo@urmc.rochester.edu.

Classifications MeSH