Structure and mechanism of the osmoregulated choline transporter BetT.

Membrane Transport Proteins / metabolism Choline / metabolism Bacterial Proteins / metabolism Cryoelectron Microscopy Pseudomonas syringae / metabolism Models, Molecular Osmoregulation Osmotic Pressure Betaine / metabolism Protein Conformation Protein Binding Structure-Activity Relationship Protein Domains

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
16 Aug 2024
Historique:
medline: 14 8 2024
pubmed: 14 8 2024
entrez: 14 8 2024
Statut: ppublish

Résumé

The choline-glycine betaine pathway plays an important role in bacterial survival in hyperosmotic environments. Osmotic activation of the choline transporter BetT promotes the uptake of external choline for synthesizing the osmoprotective glycine betaine. Here, we report the cryo-electron microscopy structures of

Identifiants

DOI: 10.1126/sciadv.ado6229 PMID: 39141726
pubmed: 39141726
doi: 10.1126/sciadv.ado6229
doi:

Substances chimiques

Membrane Transport Proteins 0
Choline N91BDP6H0X
choline transporter 0
Bacterial Proteins 0
Betaine 3SCV180C9W

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eado6229

Auteurs

Tianjiao Yang (T)

CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
University of Chinese Academy of Sciences, Beijing 101408, China.
ORCID: 0009-0009-5676-5006

Yuwei Nian (Y)

CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
ORCID: 0009-0009-0172-0038

Huajian Lin (H)

CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
ORCID: 0000-0002-9847-0996

Jing Li (J)

School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
ORCID: 0009-0003-2593-829X

Xiang Lin (X)

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China.

Tianming Li (T)

CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.

Ruiying Wang (R)

CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
ORCID: 0009-0001-4820-1843

Longfei Wang (L)

School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
ORCID: 0000-0002-7788-2123

Gwyn A Beattie (GA)

Department of Plant Pathology, Entomology and Microbiology, Iowa State University, Ames, IA 50011, USA.
ORCID: 0000-0002-0531-6843

Jinru Zhang (J)

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China.
ORCID: 0009-0004-5131-2992

Minrui Fan (M)

CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
Key Laboratory of Plant Carbon Capture, Chinese Academy of Sciences, Shanghai 200032, China.
ORCID: 0009-0002-8375-6972

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Composés chimiques organiques › Composés onium › Composés d'ammonium quaternaire › Choline › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Osmorégulation › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Phénomènes chimiques › Perméabilité › Pression osmotique › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Composés chimiques organiques › Composés onium › Composés d'ammonium quaternaire › Bétaïne › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Relation structure-activité › Structure and mechanism of the osmoregulated...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Structure and mechanism of the osmoregulated...