Exploration of microRNAs as transcriptional regulator in mumps virus infection through computational studies.

MicroRNAs / genetics Mumps / virology Humans Mumps virus / genetics Computational Biology / methods Binding Sites RNA, Messenger / genetics Molecular Docking Simulation Gene Expression Regulation Female RNA, Viral / genetics Male
Docking studies Mumps virus RNA composer RNA22 RStudio miRBase miRNA miRanda psRNATarget

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
14 08 2024
Historique:
received: 12 01 2024
accepted: 15 07 2024
medline: 15 8 2024
pubmed: 15 8 2024
entrez: 14 8 2024
Statut: epublish

Résumé

Mumps is a common childhood infection caused by the mumps virus (MuV). Aseptic meningitis and encephalitis are usual symptoms of mumps together with orchitis and oophoritis that can arise in males and females, respectively. We have used computational tools: RNA22, miRanda and psRNATarget to predict the microRNA-mRNA binding sites to find the putative microRNAs playing role in the host response to mumps virus infection. Our computational studies indicate that hsa-mir-3155a is most likely involved in mumps infection. This was further investigated by the prediction of binding sites of hsa-mir-3155a to the MuV genome. Additionally, structure prediction using MC-Fold and MC-Sym, respectively has been applied to predict the 3D structures of miRNA and mRNA. The miRNA-mRNA interaction profile between has been confirmed through molecular docking simulation studies. Taken together, the putative miRNA (hsa_miR_6794_5p) has been found to be most likely involved in the regulation of transcriptional activity in the MuV infection.

Identifiants

DOI: 10.1038/s41598-024-67717-z PMID: 39143101
pubmed: 39143101
doi: 10.1038/s41598-024-67717-z
pii: 10.1038/s41598-024-67717-z
doi:

Substances chimiques

MicroRNAs 0
RNA, Messenger 0
RNA, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

18850

Informations de copyright

© 2024. The Author(s).

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Auteurs

Mubashir Hassan (M)

The Steve and Cindy Rasmussen Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA.

Saba Shahzadi (S)

The Steve and Cindy Rasmussen Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA.

Muhammad Shahzad Iqbal (MS)

Department of Biochemistry, University of Okara, Okara, Pakistan.

Zainab Yaseeen (Z)

Department of Biotechnology, Faculty of Science and Technology (FOST), University of Central Punjab, Johar Town, Lahore, Pakistan.

Andrzej Kloczkowski (A)

The Steve and Cindy Rasmussen Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA. kloczkowski.1@osu.edu.
Department of Pediatrics, The Ohio State University, Columbus, OH, USA. kloczkowski.1@osu.edu.
Department of Biomedical Informatics, The Ohio State University, Columbus, OH, 43210, USA. kloczkowski.1@osu.edu.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Maladies du système stomatognathique › Maladies de la bouche › Maladies de la glande salivaire › Maladies de la glande parotide › Oreillons › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus › Virus des oreillons › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Sciences de l'information › Informatique › Biologie informatique › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Exploration of microRNAs as transcriptional...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Exploration of microRNAs as transcriptional...