Identification of regulatory networks and crosstalk factors in brown adipose tissue and liver of a cold-exposed cardiometabolic mouse model.
Animals
Adipose Tissue, Brown
/ metabolism
Liver
/ metabolism
Disease Models, Animal
Mice, Knockout
Cold Temperature
Gene Regulatory Networks
Energy Metabolism
/ genetics
Proteomics
Receptors, LDL
/ genetics
Signal Transduction
Male
Fibrinogen
/ metabolism
Mice, Inbred C57BL
MicroRNAs
/ metabolism
Fibronectins
/ metabolism
Transcription Factors
/ genetics
Mice
Gene Expression Regulation
Protein Interaction Maps
Brown adipose tissue
Cardiometabolic diseases
Cold exposure
Ldlr-deficient mice
Liver
Untargeted proteomics
Journal
Cardiovascular diabetology
ISSN: 1475-2840
Titre abrégé: Cardiovasc Diabetol
Pays: England
ID NLM: 101147637
Informations de publication
Date de publication:
14 Aug 2024
14 Aug 2024
Historique:
received:
30
04
2024
accepted:
07
08
2024
medline:
15
8
2024
pubmed:
15
8
2024
entrez:
14
8
2024
Statut:
epublish
Résumé
Activation of brown adipose tissue (BAT) has gained attention due to its ability to dissipate energy and counteract cardiometabolic diseases (CMDs). This study investigated the consequences of cold exposure on the BAT and liver proteomes of an established CMD mouse model based on LDL receptor-deficient (LdlrKO) mice fed a high-fat, high-sucrose, high-cholesterol diet for 16 weeks. We analyzed energy metabolism in vivo and performed untargeted proteomics on BAT and liver of LdlrKO mice maintained at 22 °C or 5 °C for 7 days. We identified several dysregulated pathways, miRNAs, and transcription factors in BAT and liver of cold-exposed Ldlrko mice that have not been previously described in this context. Networks of regulatory interactions based on shared downstream targets and analysis of ligand-receptor pairs identified fibrinogen alpha chain (FGA) and fibronectin 1 (FN1) as potential crosstalk factors between BAT and liver in response to cold exposure. Importantly, genetic variations in the genes encoding FGA and FN1 have been associated with cardiometabolic-related phenotypes and traits in humans. This study describes the key factors, pathways, and regulatory networks involved in the crosstalk between BAT and the liver in a cold-exposed CMD mouse model. These findings may provide a basis for future studies aimed at testing whether molecular mediators, as well as regulatory and signaling mechanisms involved in tissue adaption upon cold exposure, could represent a target in cardiometabolic disorders.
Sections du résumé
BACKGROUND
BACKGROUND
Activation of brown adipose tissue (BAT) has gained attention due to its ability to dissipate energy and counteract cardiometabolic diseases (CMDs).
METHODS
METHODS
This study investigated the consequences of cold exposure on the BAT and liver proteomes of an established CMD mouse model based on LDL receptor-deficient (LdlrKO) mice fed a high-fat, high-sucrose, high-cholesterol diet for 16 weeks. We analyzed energy metabolism in vivo and performed untargeted proteomics on BAT and liver of LdlrKO mice maintained at 22 °C or 5 °C for 7 days.
RESULTS
RESULTS
We identified several dysregulated pathways, miRNAs, and transcription factors in BAT and liver of cold-exposed Ldlrko mice that have not been previously described in this context. Networks of regulatory interactions based on shared downstream targets and analysis of ligand-receptor pairs identified fibrinogen alpha chain (FGA) and fibronectin 1 (FN1) as potential crosstalk factors between BAT and liver in response to cold exposure. Importantly, genetic variations in the genes encoding FGA and FN1 have been associated with cardiometabolic-related phenotypes and traits in humans.
DISCUSSION
CONCLUSIONS
This study describes the key factors, pathways, and regulatory networks involved in the crosstalk between BAT and the liver in a cold-exposed CMD mouse model. These findings may provide a basis for future studies aimed at testing whether molecular mediators, as well as regulatory and signaling mechanisms involved in tissue adaption upon cold exposure, could represent a target in cardiometabolic disorders.
Identifiants
pubmed: 39143620
doi: 10.1186/s12933-024-02397-7
pii: 10.1186/s12933-024-02397-7
doi:
Substances chimiques
Receptors, LDL
0
Fibrinogen
9001-32-5
MicroRNAs
0
Fibronectins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
298Subventions
Organisme : Progetti di Rilevante Interesse Nazionale
ID : PRIN 2022 7KTSAT
Organisme : Ricerca Finalizzata, Ministry of Health
ID : RF-2019-12370896
Organisme : Nanokos
ID : EUROPEAID/173691/DD/ACT/XK
Organisme : PNRR Missione 4
ID : Progetto MUSA
Organisme : PNRR Missione 6
ID : PNRR-MAD-2022-12375913
Organisme : CARDINNOV, Ministry of Research and University under the umbrella of the Partnership Fostering a European Research Area for Health (ERA4Health)
ID : GA N° 101095426
Organisme : Austrian Science Fund
ID : 10.55776/P32400
Organisme : Medizinische Universität Graz
ID : VascHealth
Organisme : Amt der Steiermärkischen Landesregierung
ID : add-on funding F73
Organisme : City of Graz
ID : add-on funding F73
Informations de copyright
© 2024. The Author(s).
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