Dysregulation of Porphyromonas gingivalis Agmatine Deiminase Expression in Alzheimer's Disease.

Alzheimer’s disease Periodontitis Porphyromonas gingivalis arginine polyamines metabolism saliva.

Journal

Current Alzheimer research
ISSN: 1875-5828
Titre abrégé: Curr Alzheimer Res
Pays: United Arab Emirates
ID NLM: 101208441

Informations de publication

Date de publication:
13 Aug 2024
Historique:
received: 26 05 2024
revised: 20 07 2024
accepted: 23 07 2024
medline: 15 8 2024
pubmed: 15 8 2024
entrez: 15 8 2024
Statut: aheadofprint

Résumé

Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder, with a significant burden on global health. AD is characterized by a progressive cognitive decline and memory loss. Emerging research suggests a potential link between periodontitis, specifically the presence of oral bacteria such as Porphyromonas gingivalis (P. gingivalis), and AD progression. P. gingivalis produces an enzyme, Agmatine deiminase (AgD), which converts agmatine to N-carbamoyl putrescine (NCP), serving as a precursor to essential polyamines. Recent studies have confirmed the correlation between disruptions in polyamine metabolism and cognitive impairment. This study aims to investigate the dysregulation of P. gingivalis Agmatine deiminase (PgAgD) in the context of AD. Saliva samples were collected from a total of 54 individuals, including 27 AD patients and 27 healthy controls. The expression of the PgAgD gene was analyzed using quantitative Real-- Time PCR. The results showed a significant decrease in PgAgD gene expression in the saliva samples of AD patients compared to healthy controls. This downregulation was found in AD patients with advanced stages of periodontitis. Additionally, a correlation was observed between the decrease in PgAgD expression and the 30-item Mini-Mental State Examination (MMSE) score. These findings suggest that measuring PgAgD expression in saliva could be a noninvasive tool for monitoring AD progression and aid in the early diagnosis of patients with periodontitis. Further research is needed to validate our results and explore the underlying mechanisms linking periodontitis, PgAgD expression, and AD pathophysiology.

Sections du résumé

BACKGROUND BACKGROUND
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder, with a significant burden on global health. AD is characterized by a progressive cognitive decline and memory loss. Emerging research suggests a potential link between periodontitis, specifically the presence of oral bacteria such as Porphyromonas gingivalis (P. gingivalis), and AD progression. P. gingivalis produces an enzyme, Agmatine deiminase (AgD), which converts agmatine to N-carbamoyl putrescine (NCP), serving as a precursor to essential polyamines. Recent studies have confirmed the correlation between disruptions in polyamine metabolism and cognitive impairment.
OBJECTIVE OBJECTIVE
This study aims to investigate the dysregulation of P. gingivalis Agmatine deiminase (PgAgD) in the context of AD.
METHODS METHODS
Saliva samples were collected from a total of 54 individuals, including 27 AD patients and 27 healthy controls. The expression of the PgAgD gene was analyzed using quantitative Real-- Time PCR.
RESULTS RESULTS
The results showed a significant decrease in PgAgD gene expression in the saliva samples of AD patients compared to healthy controls. This downregulation was found in AD patients with advanced stages of periodontitis. Additionally, a correlation was observed between the decrease in PgAgD expression and the 30-item Mini-Mental State Examination (MMSE) score.
CONCLUSION CONCLUSIONS
These findings suggest that measuring PgAgD expression in saliva could be a noninvasive tool for monitoring AD progression and aid in the early diagnosis of patients with periodontitis. Further research is needed to validate our results and explore the underlying mechanisms linking periodontitis, PgAgD expression, and AD pathophysiology.

Identifiants

DOI: 10.2174/0115672050327009240808103542 PMID: 39143870
pubmed: 39143870
pii: CAR-EPUB-142264
doi: 10.2174/0115672050327009240808103542
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

Auteurs

Asma Hamdi (A)

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.

Sana Baroudi (S)

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.

Alya Gharbi (A)

Neurology Department, Razi University Hospital, Manouba, Tunisia.
Faculty of Medicine of Tunis, University of Tunis El Manar, La Rabta, Tunis, Tunisia.
Clinical Investigation Center (CIC), Razi University Hospital, Manouba, Tunisia.

Wafa Babay (W)

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.

Ahmed Baligh Laaribi (AB)

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.

Imene Kacem (I)

Neurology Department, Razi University Hospital, Manouba, Tunisia.
Faculty of Medicine of Tunis, University of Tunis El Manar, La Rabta, Tunis, Tunisia.
Clinical Investigation Center (CIC), Razi University Hospital, Manouba, Tunisia.

Saloua Mrabet (S)

Neurology Department, Razi University Hospital, Manouba, Tunisia.
Faculty of Medicine of Tunis, University of Tunis El Manar, La Rabta, Tunis, Tunisia.
Clinical Investigation Center (CIC), Razi University Hospital, Manouba, Tunisia.

Ines Zidi (I)

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.

Naouel Klibi (N)

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.

Riadh Gouider (R)

Neurology Department, Razi University Hospital, Manouba, Tunisia.
Faculty of Medicine of Tunis, University of Tunis El Manar, La Rabta, Tunis, Tunisia.
Clinical Investigation Center (CIC), Razi University Hospital, Manouba, Tunisia.

Hadda-Imene Ouzari (HI)

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar, Campus Universitaire 2092, Tunis, Tunisia.

Classifications MeSH