The network interactions between the porcine deltacoronavirus nucleocapsid protein and host cellular proteins.

GO analysis Interaction network KEGG analysis Nucleocapsid protein Porcine deltacoronavirus

Journal

Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469

Informations de publication

Date de publication:
17 Aug 2024
Historique:
received: 05 07 2024
revised: 14 08 2024
accepted: 15 08 2024
medline: 19 8 2024
pubmed: 19 8 2024
entrez: 18 8 2024
Statut: aheadofprint

Résumé

Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus that can cause diarrhea in pigs of all ages with varying severity. Host-virus protein interactions are critical for intracellular viral replication. Elucidating the interactions between cellular and viral proteins can help us to design antiviral strategies. PDCoV N protein is the most abundant and vital regulator in virus replication. In this study, 604 host proteins were identified to interact with PDCoV N protein by Co-IP combined with LC-MS, of which 243 proteins were specifically bound to N protein. PPI analysis revealed that the N-interacting host proteins are categorized into three groups: ribonucleoprotein complex biogenesis modulation, cellular nitrogen compound metabolism, and nucleic acid binding. GO and KEGG analyses showed that the host proteins are primarily involved in mRNA splicing, stress granule assembly, spliceosomal snRNP assembly. Additionally, four host proteins-TRIM25, HNRNPUL1, RPS27A, and SLC3A2-were selected to validate the interactome data through Co-IP and Confocal assays. This study can help in designing anti-PDCoV strategies and understanding the replication mechanism of PDCoV.

Identifiants

DOI: 10.1016/j.vetmic.2024.110225 PMID: 39154555
pubmed: 39154555
pii: S0378-1135(24)00247-5
doi: 10.1016/j.vetmic.2024.110225
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110225

Informations de copyright

Copyright © 2024 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no competing interests.

Auteurs

Hui Jiang (H)

Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China; Department of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, China.

Mengle Jia (M)

Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China; College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.

Jiaqi Xiong (J)

Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China; College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.

Changrun Zhao (C)

College of Animal Science and Technology, Guangxi University, Nanning 530004, China.

Ting Wang (T)

Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China; College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.

Lingbao Kong (L)

Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China; College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.

Qi Peng (Q)

Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China; College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address: qipeng113@126.com.

Classifications MeSH