Monitoring GPCR conformation with GFP-inspired dyes.

Biochemistry Biophysics Structural biology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
16 Aug 2024
Historique:
received: 12 02 2024
revised: 15 05 2024
accepted: 02 07 2024
medline: 19 8 2024
pubmed: 19 8 2024
entrez: 19 8 2024
Statut: epublish

Résumé

Solvatochromic compounds have emerged as valuable environment-sensitive probes for biological research. Here we used thiol-reactive solvatochromic analogs of the green fluorescent protein (GFP) chromophore to track conformational changes in two proteins, recoverin and the A

Identifiants

DOI: 10.1016/j.isci.2024.110466 PMID: 39156645 PMC: PMC11326922
pubmed: 39156645
doi: 10.1016/j.isci.2024.110466
pii: S2589-0042(24)01691-2
pmc: PMC11326922
doi:

Types de publication

Journal Article

Langues

eng

Pagination

110466

Informations de copyright

© 2024 The Authors. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Auteurs

Anatoliy Belousov (A)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.

Ivan Maslov (I)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.
Dynamic Bioimaging Lab, Advanced Optical Microscopy Centre, Biomedical Research Institute, Agoralaan C (BIOMED), Hasselt University, 3590 Diepenbeek, Belgium.
Laboratory for Photochemistry and Spectroscopy, Division for Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, 3001 Leuven, Belgium.

Philipp Orekhov (P)

Faculty of Biology, Shenzhen MSU-BIT University, Shenzhen 518172, China.
Sechenov University, Moscow 119146, Russia.

Polina Khorn (P)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.

Pavel Kuzmichev (P)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.

Nadezhda Baleeva (N)

Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Pirogov Russian National Research Medical University, Moscow 117997, Russia.

Vladislav Motov (V)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.

Andrey Bogorodskiy (A)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.

Svetlana Krasnova (S)

Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
National Research University Higher School of Economics, Moscow 101000, Russia.

Konstantin Mineev (K)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.

Dmitry Zinchenko (D)

Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia.

Evgeni Zernii (E)

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.

Valentin Ivanovich (V)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.

Sergei Permyakov (S)

Institute for Biological Instrumentation, Russian Academy of Sciences, Pushchino 142292, Russia.

Johan Hofkens (J)

Laboratory for Photochemistry and Spectroscopy, Division for Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, 3001 Leuven, Belgium.
Max Plank Institute for Polymer Research, Mainz, Germany.

Jelle Hendrix (J)

Dynamic Bioimaging Lab, Advanced Optical Microscopy Centre, Biomedical Research Institute, Agoralaan C (BIOMED), Hasselt University, 3590 Diepenbeek, Belgium.
Laboratory for Photochemistry and Spectroscopy, Division for Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, 3001 Leuven, Belgium.

Vadim Cherezov (V)

Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.

Thomas Gensch (T)

Laboratory for Photochemistry and Spectroscopy, Division for Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, 3001 Leuven, Belgium.

Alexander Mishin (A)

Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.

Mikhail Baranov (M)

Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Pirogov Russian National Research Medical University, Moscow 117997, Russia.

Alexey Mishin (A)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.

Valentin Borshchevskiy (V)

Moscow Institute of Physics and Technology, Dolgoprudny 141701, Russia.
Joint Institute for Nuclear Research, Dubna 141980, Russian Federation.

Classifications MeSH