Effects of fluid shear stress on oral biofilm formation and composition and the transcriptional response of Streptococcus gordonii.
Streptococcus gordonii
biofilms
oral
shear
Journal
Molecular oral microbiology
ISSN: 2041-1014
Titre abrégé: Mol Oral Microbiol
Pays: Denmark
ID NLM: 101524770
Informations de publication
Date de publication:
19 Aug 2024
19 Aug 2024
Historique:
revised:
19
06
2024
received:
07
02
2024
accepted:
23
07
2024
medline:
19
8
2024
pubmed:
19
8
2024
entrez:
19
8
2024
Statut:
aheadofprint
Résumé
Biofilms are subjected to many environmental pressures that can influence community structure and physiology. In the oral cavity, and many other environments, biofilms are exposed to forces generated by fluid flow; however, our understanding of how oral biofilms respond to these forces remains limited. In this study, we developed a linear rocker model of fluid flow to study the impact of shear forces on Streptococcus gordonii and dental plaque-derived multispecies biofilms. We observed that as shear forces increased, S. gordonii biofilm biomass decreased. Reduced biomass was largely independent of overall bacterial growth. Transcriptome analysis of S. gordonii biofilms exposed to moderate levels of shear stress uncovered numerous genes with differential expression under shear. We also evaluated an ex vivo plaque biofilm exposed to fluid shear forces. Like S. gordonii, the plaque biofilm displayed decreased biomass as shear forces increased. Examination of plaque community composition revealed decreased diversity and compositional changes in the plaque biofilm exposed to shear. These studies help to elucidate the impact of fluid shear on oral bacteria and may be extended to other bacterial biofilm systems.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE025618
Pays : United States
Organisme : NIDCR NIH HHS
ID : K08 DE027705
Pays : United States
Organisme : NIDCR NIH HHS
ID : R03 DE031337
Pays : United States
Organisme : National Science Foundation
ID : 2236497
Informations de copyright
© 2024 The Author(s). Molecular Oral Microbiology published by John Wiley & Sons Ltd.
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