Extracellular production of azurin by reusable magnetic Fe
Pseudomonas aeruginosa
azurin
extracellular secretion
immobilization
magnetic nanoparticles
Journal
Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927
Informations de publication
Date de publication:
17 Aug 2024
17 Aug 2024
Historique:
received:
16
04
2024
revised:
01
08
2024
accepted:
15
08
2024
medline:
20
8
2024
pubmed:
20
8
2024
entrez:
19
8
2024
Statut:
aheadofprint
Résumé
Azurin, found in the periplasm of Pseudomonas aeruginosa, has garnered significant attention as a potential anticancer agent in recent years. High-level secretion of proteins into the culture medium, offers a significant advantage over periplasmic or cytoplasmic expression. In this study, for the first time, P. aeruginosa cells were immobilized with magnetic nanoparticles (MNPs) to ensure effective, simple and quick separation of the cells and secretion of periplasmic azurin protein to the culture medium. For this purpose, polyethyleneimine-coated iron oxide (Fe
Identifiants
pubmed: 39159754
pii: S0168-1656(24)00226-8
doi: 10.1016/j.jbiotec.2024.08.010
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2024 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. CONFLICT OF INTEREST The authors declare no conflict of interest.