Development of a novel bacterial production system for recombinant bioactive proteins completely free from endotoxin contamination.

Acinetobacter baumannii VHH endotoxin recombinant protein expression

Journal

PNAS nexus
ISSN: 2752-6542
Titre abrégé: PNAS Nexus
Pays: England
ID NLM: 9918367777906676

Informations de publication

Date de publication:
Aug 2024
Historique:
received: 18 02 2024
accepted: 29 07 2024
medline: 20 8 2024
pubmed: 20 8 2024
entrez: 20 8 2024
Statut: epublish

Résumé

Endotoxins, or lipopolysaccharides (LPS), are potent immunostimulatory molecules of critical concern in bacterial recombinant protein expression systems. The gram-negative bacterium

Identifiants

DOI: 10.1093/pnasnexus/pgae328 PMID: 39161731 PMC: PMC11331542
pubmed: 39161731
doi: 10.1093/pnasnexus/pgae328
pii: pgae328
pmc: PMC11331542
doi:

Types de publication

Journal Article

Langues

eng

Pagination

pgae328

Informations de copyright

© The Author(s) 2024. Published by Oxford University Press on behalf of National Academy of Sciences.

Auteurs

Go Kamoshida (G)

Department of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
ORCID: 0000-0001-6300-0911

Daiki Yamaguchi (D)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
ORCID: 0009-0009-5581-7089

Yuki Kaya (Y)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
ORCID: 0009-0003-3926-7079

Toshiki Yamakado (T)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

Kenta Yamashita (K)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

Moe Aoyagi (M)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

Saaya Nagai (S)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

Noriteru Yamada (N)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

Yu Kawagishi (Y)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

Mizuki Sugano (M)

Department of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.

Yoshiaki Sakairi (Y)

Department of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.

Mikako Ueno (M)

Department of Intractable Diseases, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjyuku-ku, Tokyo 162-8655, Japan.

Norihiko Takemoto (N)

Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjyuku-ku, Tokyo 162-8655, Japan.

Yuji Morita (Y)

Department of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
ORCID: 0000-0003-1371-8591

Yukihito Ishizaka (Y)

Department of Intractable Diseases, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjyuku-ku, Tokyo 162-8655, Japan.
ORCID: 0000-0002-1863-6096

Kinnosuke Yahiro (K)

Laboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
ORCID: 0000-0002-9813-0780

Classifications MeSH