Rapid single-tier serodiagnosis of Lyme disease.

Lyme Disease / diagnosis Humans Serologic Tests / methods Borrelia burgdorferi / immunology Antibodies, Bacterial / blood Sensitivity and Specificity Immunoglobulin M / blood Immunoglobulin G / blood Antigens, Bacterial / immunology Machine Learning Epitopes / immunology Point-of-Care Testing Point-of-Care Systems

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
20 Aug 2024
Historique:
received: 21 07 2023
accepted: 29 07 2024
medline: 21 8 2024
pubmed: 21 8 2024
entrez: 20 8 2024
Statut: epublish

Résumé

Point-of-care serological and direct antigen testing offers actionable insights for diagnosing challenging illnesses, empowering distributed health systems. Here, we report a POC-compatible serologic test for Lyme disease (LD), leveraging synthetic peptides specific to LD antibodies and a paper-based platform for rapid, and cost-effective diagnosis. Antigenic epitopes conserved across Borrelia burgdorferi genospecies, targeted by IgG and IgM antibodies, are selected to develop a multiplexed panel for detection of LD antibodies from patient sera. Multiple peptide epitopes, when combined synergistically with a machine learning-based diagnostic model achieve high sensitivity without sacrificing specificity. Blinded validation with 15 LD-positive and 15 negative samples shows 95.5% sensitivity and 100% specificity. Blind testing with the CDC's LD repository samples confirms the test accuracy, matching lab-based two-tier results, correctly differentiating between LD and look-alike diseases. This LD diagnostic test could potentially replace the cumbersome two-tier testing, improving diagnosis and enabling earlier treatment while facilitating immune monitoring and surveillance.

Identifiants

DOI: 10.1038/s41467-024-51067-5 PMID: 39164226
pubmed: 39164226
doi: 10.1038/s41467-024-51067-5
pii: 10.1038/s41467-024-51067-5
doi:

Substances chimiques

Antibodies, Bacterial 0
Immunoglobulin M 0
Immunoglobulin G 0
Antigens, Bacterial 0
Epitopes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

7124

Subventions

Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : R44AI150060
Organisme : National Science Foundation (NSF)
ID : 1648451

Informations de copyright

© 2024. The Author(s).

Références

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Auteurs

Rajesh Ghosh (R)

Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.
ORCID: 0000-0002-7408-8944

Hyou-Arm Joung (HA)

Electrical & Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA.
California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA.
ORCID: 0000-0001-5106-4459

Artem Goncharov (A)

Electrical & Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA.
California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA.

Barath Palanisamy (B)

Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.

Kevin Ngo (K)

Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.

Katarina Pejcinovic (K)

Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.
ORCID: 0009-0002-7802-3993

Nicole Krockenberger (N)

Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.
ORCID: 0009-0001-1693-1120

Elizabeth J Horn (EJ)

Lyme Disease Biobank, Portland, Oregon, OR, 97221, USA.

Omai B Garner (OB)

Department of Pathology and Laboratory Medicine, University of California, Los Angeles, CA, 90095, USA.

Ezdehar Ghazal (E)

Department of Pathology, Microbiology, and Immunology, New York Medical College, Valhalla, NY, 10595, USA.

Andrew O'Kula (A)

Department of Pathology, Microbiology, and Immunology, New York Medical College, Valhalla, NY, 10595, USA.

Paul M Arnaboldi (PM)

Department of Pathology, Microbiology, and Immunology, New York Medical College, Valhalla, NY, 10595, USA.
Biopeptides, Corp, Ridgefield, CT, 06877, USA.

Raymond J Dattwyler (RJ)

Department of Pathology, Microbiology, and Immunology, New York Medical College, Valhalla, NY, 10595, USA.
Biopeptides, Corp, Ridgefield, CT, 06877, USA.
ORCID: 0000-0002-1983-1301

Aydogan Ozcan (A)

Bioengineering Department, University of California, Los Angeles, CA, 90095, USA. ozcan@ucla.edu.
Electrical & Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA. ozcan@ucla.edu.
California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA. ozcan@ucla.edu.
Department of Surgery, University of California, Los Angeles, CA, 90095, USA. ozcan@ucla.edu.
ORCID: 0000-0002-0717-683X

Dino Di Carlo (D)

Bioengineering Department, University of California, Los Angeles, CA, 90095, USA. dicarlo@ucla.edu.
California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA. dicarlo@ucla.edu.
Department of Mechanical Engineering, University of California, Los Angeles, CA, 90095, USA. dicarlo@ucla.edu.
ORCID: 0000-0003-3942-4284

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Classifications MeSH

questionsmedicales.fr › Infection › Maladies vectorielles › Maladies transmises par les tiques › Maladie de Lyme › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Tests sérologiques › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Bactéries › Spirochaetales › Spirochaetaceae › Borrelia › Groupe Borrelia burgdorferi › Borrelia burgdorferi › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antibactériens › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Isotypes des immunoglobulines › Immunoglobuline M › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Isotypes des immunoglobulines › Immunoglobuline G › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes bactériens › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Algorithmes › Intelligence artificielle › Apprentissage machine › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Administration des services de santé › Gestion des soins aux patients › Systèmes automatisés lit malade › Analyse sur le lieu d'intervention › Rapid single-tier serodiagnosis of Lyme disease.
questionsmedicales.fr › Administration des services de santé › Gestion des soins aux patients › Systèmes automatisés lit malade › Rapid single-tier serodiagnosis of Lyme disease.