Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 Aug 2024
21 Aug 2024
Historique:
received:
05
07
2023
accepted:
08
08
2024
medline:
22
8
2024
pubmed:
22
8
2024
entrez:
21
8
2024
Statut:
epublish
Résumé
For accurate mitotic cell division, replicated chromatin must be assembled into chromosomes and faithfully segregated into daughter cells. While protein factors like condensin play key roles in this process, it is unclear how chromosome assembly proceeds as molecular events of nucleosomes in living cells and how condensins act on nucleosomes to organize chromosomes. To approach these questions, we investigate nucleosome behavior during mitosis of living human cells using single-nucleosome tracking, combined with rapid-protein depletion technology and computational modeling. Our results show that local nucleosome motion becomes increasingly constrained during mitotic chromosome assembly, which is functionally distinct from condensed apoptotic chromatin. Condensins act as molecular crosslinkers, locally constraining nucleosomes to organize chromosomes. Additionally, nucleosome-nucleosome interactions via histone tails constrain and compact whole chromosomes. Our findings elucidate the physical nature of the chromosome assembly process during mitosis.
Identifiants
pubmed: 39169041
doi: 10.1038/s41467-024-51454-y
pii: 10.1038/s41467-024-51454-y
doi:
Substances chimiques
Nucleosomes
0
condensin complexes
0
DNA-Binding Proteins
0
Adenosine Triphosphatases
EC 3.6.1.-
Multiprotein Complexes
0
Chromatin
0
Histones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7152Subventions
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP20H05936
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21H02453
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP24H00061
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP23K17398
Informations de copyright
© 2024. The Author(s).
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