RNA sequestration in P-bodies sustains myeloid leukaemia.
Journal
Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575
Informations de publication
Date de publication:
21 Aug 2024
21 Aug 2024
Historique:
received:
18
09
2023
accepted:
18
07
2024
medline:
22
8
2024
pubmed:
22
8
2024
entrez:
21
8
2024
Statut:
aheadofprint
Résumé
Post-transcriptional mechanisms are fundamental safeguards of progenitor cell identity and are often dysregulated in cancer. Here, we identified regulators of P-bodies as crucial vulnerabilities in acute myeloid leukaemia (AML) through genome-wide CRISPR screens in normal and malignant haematopoietic progenitors. We found that leukaemia cells harbour aberrantly elevated numbers of P-bodies and show that P-body assembly is crucial for initiation and maintenance of AML. Notably, P-body loss had little effect upon homoeostatic haematopoiesis but impacted regenerative haematopoiesis. Molecular characterization of P-bodies purified from human AML cells unveiled their critical role in sequestering messenger RNAs encoding potent tumour suppressors from the translational machinery. P-body dissolution promoted translation of these mRNAs, which in turn rewired gene expression and chromatin architecture in leukaemia cells. Collectively, our findings highlight the contrasting and unique roles of RNA sequestration in P-bodies during tissue homoeostasis and oncogenesis. These insights open potential avenues for understanding myeloid leukaemia and future therapeutic interventions.
Identifiants
pubmed: 39169219
doi: 10.1038/s41556-024-01489-6
pii: 10.1038/s41556-024-01489-6
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Cancer Prevention and Research Institute of Texas (Cancer Prevention Research Institute of Texas)
ID : RR200079
Organisme : Cancer Prevention and Research Institute of Texas (Cancer Prevention Research Institute of Texas)
ID : RR200040
Organisme : U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health (OER)
ID : 1R35GM147126-01
Organisme : U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health (OER)
ID : 1F32CA288043-01
Organisme : U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health (OER)
ID : 5T32DK060445-19
Organisme : Ministry of Economy and Competitiveness | Instituto de Salud Carlos III (Institute of Health Carlos III)
ID : CP22/00127
Organisme : Ministry of Economy and Competitiveness | Instituto de Salud Carlos III (Institute of Health Carlos III)
ID : CP19/00176
Organisme : Worldwide Cancer Research
ID : 20-0269
Pays : United Kingdom
Organisme : Austrian Science Fund (Fonds zur Förderung der Wissenschaftlichen Forschung)
ID : P-35628
Organisme : Austrian Science Fund (Fonds zur Förderung der Wissenschaftlichen Forschung)
ID : P35298
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature Limited.
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