Entomopathogenic Bacillus cereus impairs the fitness of the spotted-wing drosophila, Drosophila suzukii.
DUOX‐ROS‐JNK pathway
entomopathogen
intestinal integrity
pest biocontrol
the spotted‐wing fly
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
21 Aug 2024
21 Aug 2024
Historique:
revised:
22
07
2024
received:
04
05
2024
accepted:
23
07
2024
medline:
22
8
2024
pubmed:
22
8
2024
entrez:
22
8
2024
Statut:
aheadofprint
Résumé
Drosophila suzukii is a notorious pest which causes devastating damage to thin-skinned fruits, and the larvae feed on the fruit, causing extensive agricultural economic loss. The current application of insecticides to manage this pest results in serious resistance and environmental hazards, so an alternative strategy for D. suzukii biocontrol is urgently needed. Here, we reported that entomopathogenic Bacillus cereus has the potential to biocontrol D. suzukii. We isolated and identified the bacterial strain, B. cereus H1, that was detrimental to the fitness of both D. suzukii progenies and parents. D. suzukii was robustly repelled to depositing eggs on the halves with metabolites of B. cereus H1. Both males and females of D. suzukii were susceptible to B. cereus H1. B. cereus H1 significantly arrested larval development with at least 40% lethal larvae. The median lethal time (LT50) of males and females of D. suzukii challenged with B. cereus H1 was 3 and 2 d, respectively. Moreover, B. cereus H1 disrupted the intestinal integrity and pH value of D. suzukii and resulted in an increase in bacterial load of guts and hemolymph. Mechanistically, infection of B. cereus H1 led to the activation of the dual oxidase (DUOX)-ROS-Jun N-terminal kinase (JNK) pathway. The findings showed that the entomopathogen B. cereus H1 could potentially act as a biological control agent against D. suzukii, advancing fundamental concepts of integrated pest management programs against D. suzukii.
Identifiants
pubmed: 39169715
doi: 10.1111/1744-7917.13439
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Grants of Anhui Natural Science Foundation
Organisme : National Natural Science Foundation of China
Organisme : Talent Grants of Anhui Agricultural University
Informations de copyright
© 2024 Institute of Zoology, Chinese Academy of Sciences.
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